Human induced pluripotent stem cells (hiPSCs) offer a unique opportunity for disease modeling. However, it is not invariably successful to recapitulate the disease phenotype because of the immaturity of hiPSC-derived cardiomyocytes (hiPSC-CMs). The purpose of this study was to establish and analyze iPSC-based model of catecholaminergic polymorphic ventricular tachycardia (CPVT), which is characterized by adrenergically mediated lethal arrhythmias, more precisely using electrical pacing that could promote the development of new pharmacotherapies.
We generated hiPSCs from a 37-year-old CPVT patient and differentiated them into cardiomyocytes. Under spontaneous beating conditions, no significant difference was found in the timing irregularity of spontaneous Ca2+ transients between control- and CPVT-hiPSC-CMs. Using Ca2+ imaging at 1 Hz electrical field stimulation, isoproterenol induced an abnormal diastolic Ca2+ increase more frequently in CPVT- than in control-hiPSC-CMs (control 12% vs. CPVT 43%, p<0.05). Action potential recordings of spontaneous beating hiPSC-CMs revealed no significant difference in the frequency of delayed afterdepolarizations (DADs) between control and CPVT cells. After isoproterenol application with pacing at 1 Hz, 87.5% of CPVT-hiPSC-CMs developed DADs, compared to 30% of control-hiPSC-CMs (p<0.05). Pre-incubation with 10 μM S107, which stabilizes the closed state of the ryanodine receptor 2, significantly decreased the percentage of CPVT-hiPSC-CMs presenting DADs to 25% (p<0.05).
We recapitulated the electrophysiological features of CPVT-derived hiPSC-CMs using electrical pacing. The development of DADs in the presence of isoproterenol was significantly suppressed by S107. Our model provides a promising platform to study disease mechanisms and screen drugs.
Copyright: © 2016 Sasaki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by Japan Society for the Promotion of Science KAKENHI Grant Number 25461054 and Suzuken Memorial Foundation Grant Number 10-097. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Catecholaminergic polymorphic ventricular tachycardia (CPVT) is a hereditary arrhythmic disorder characterized by bidirectional ventricular tachycardia (VT) that is triggered by emotional stress or physical exercise and leads to syncope or sudden cardiac death without structural heart disease. CPVT is caused by autosomal dominant mutations in the cardiac ryanodine receptor gene (RyR2) , the calmodulin gene (CALM1, CALM2) [2, 3] and the inward rectifying potassium channel gene (KCNJ2)  and autosomal recessive mutations in the cardiac calsequestrin gene (CASQ2)  and the triadin gene (TRDN) . Approximately 50–55% of CPVT cases are associated with RyR2 mutations  and 1–2% are due to CASQ2 mutations . Beta-blockers are the first-line therapy for CPVT, but they often fail to prevent fatal arrhythmias . Recently, flecainide, a class Ic Na+ channel blocker, has been reported to be effective for treating CPVT patients .
The advent of human induced pluripotent stem cell (hiPSC) technology has enabled us to use human cardiomyocytes that have the same genetic background as the patients. There have been several reports of CPVT iPSC-based model [11–15], however, it is becoming clearer that hiPSC-derived cardiomyocytes (hiPSC-CMs) have immature electrophysiological and structural properties compared to adult human cardiomyocytes [16, 17], which hamper us to analyze their phenotype precisely. In the present study, we demonstrated that hiPSC-CMs beat in an irregular disorganized pattern even in those of control, and suggested the usefulness of electrical pacing during Ca2+ transient and action potential (AP) recordings in hiPSC-CMs. In addition, we investigated the efficacy of S107, a 1,4-benzothiazepine derivative that is a promising candidate drug for treating CPVT. S107 was discovered by Marks et al and reported to correct leaky RyR1, 2 by stabilizing interactions between RyR 1, 2 and calstabin 1, 2 . The potency of S107 for preventing the development of DADs in CPVT-hiPSC-CMs indicates that our model could be useful for investigating new pharmacotherapies.
Human dermal fibroblasts were obtained from a patient after written informed consent was obtained. The fibroblasts were retrovirally transduced with a combination of 4 transcription factors (Oct3/4, Sox2, Klf-4, c-Myc) to generate hiPSCs. This study was approved by Kyoto University ethics review board (G259) and conformed to the Declaration of Helsinki. All patients provided written informed consent. The control hiPSC line, 201B7, was generated from a healthy individual using the same transcription factors . The hiPSCs were differentiated into cardiomyocytes using an embryoid body (EB) differentiating system described previously . For Ca2+ imaging, EBs were treated with collagenase B (Roche, Indianapolis, IN, USA) and trypsin EDTA (Nacalai Tesque, Kyoto, Japan) at day 21 of differentiation and dispersed into single cells or small clusters which were plated onto gelatin-coated dishes. For gene expression analyses, EBs were plated onto fibronectin-coated dishes without dissociation at day 21. After being plated on dishes, hiPSC-CMs were maintained in culture medium consisting of DMEM/F12 supplemented with 2% fetal bovine serum, 2 mmol/L L-glutamine, 0.1 mmol/L non-essential amino acids, 0.1 mmol/L β-mercaptoethanol, 50 U/ml penicillin, and 50 μg/ml streptomycin . The medium was renewed every 2–3 days.
Genomic DNA was isolated from control and CPVT-hiPSC lines by GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St Louis, MO, USA). Purified DNA was amplified with specific primers and analyzed by 3100 Genetic Analyzer and Big Dye Terminator v1.1 (Applied Biosystems, Foster City, CA, USA). Chromosomal G-banding analysis was performed using a standard procedure (Nihon Gene Research Laboratories, Sendai, Japan). Primers are detailed in S1 Table.
The hiPSC colonies were fixed in 4% paraformaldehyde (PFA) for 20 min. The cells were permeabilized in 0.2% Triton X-100 (Nacalai Tesque). The samples were stained with the following primary antibodies: mouse monoclonal anti-OCT3/4 (1:50; Santa Cruz Biotechnology, Delaware, CA, USA), mouse monoclonal anti-SSEA4 (1:200; Santa Cruz Biotechnology), and mouse monoclonal anti-TRA 1–60 (1:200; Santa Cruz Biotechnology). The secondary antibody was donkey anti-mouse Alexafluor 488 (1:1000, Invitrogen, Carlsbad, CA, USA). The nuclei were stained with DAPI (1:2000, Wako Pure Chemical Industries, Osaka, Japan). The specimens were observed under a fluorescence microscope (Biozero BZ-9000; KEYENCE, Osaka, Japan).
In order to determine the pluripotency of hiPSCs, we performed teratoma formation in immune-compromised mice. Two NOD/SCID male mice were maintained under a 12 hours light / 12 hours dark cycle and fed ad libitium. Mice were inspected daily by the veterinary staff. Following anesthesia with pentobarbital (50 mg/kg), the hiPSCs were injected as cell clumps into NOD/SCID mice under the testis capsule. The injection site was monitored for tumor growth weekly. Tumor samples were collected at 8 weeks, fixed in 10% formalin and stained with hematoxylin and eosin. Mice were euthanized by cervical dislocation. All animal experiments were performed in accordance with the ‘Guide for the Care and Use of Laboratory Animals’ (2011) of the National Institutes of Health and the Regulation on Animal Experimentation at Kyoto University, and approved by Ethics Committee of Kyoto University (Permit Number: kei 31–18).
Total RNA was isolated using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) from 20 to 30 spontaneously beating EBs microdissected at day 30 and day 90, and treated with TURBO DNA-free Kit (Applied Biosystems, Foster City, CA, USA). Total RNA from human whole heart tissue (BioChain Institute, Newark, CA, USA) was also reverse transcribed into complementary DNA (cDNA) for comparison. The cDNA was synthesized from 1 μg of total RNA, in a total volume of 20 μl, using oligo (dT)18 primer with Transcriptor First Strand cDNA Synthesis Kit (Roche). TaqMan real-time PCR assay was performed using FastStart Universal Probe Master (Rox) and an appropriate probe from Universal ProbeLibrary Set (Roche). The expression of genes of interest was normalized to that of GAPDH. Relative quantification was calculated according to the ΔΔCT method. The changes in gene expression levels were compared with those of adult human heart. The fold change is expressed as mean ± SEM. A list of the primers used in these experiments is provided in S1 Table.
The hiPSC-CMs were dispersed with collagenase B and Trypsin EDTA and plated onto glass coverslips coated with fibronectin (BD Biosciences, San Jose, CA, USA). After 5–7 days, dissociated hiPSC-CMs on a coverslip were loaded with 2 μmol/L Fluo-8 (AAT Bioquest, Sunnyvale, CA, USA) in the culture medium described above. After incubation for 30 min at 37°C in 5% CO2, the medium was replaced with normal tyrode solution containing (in mmol/L): 140 NaCl, 0.33 NaH2PO4, 5.4 KCl, 1.8 CaCl2, 0.5 MgCl2, 5.0 HEPES, and 5.5 D-Glucose. Spontaneously contracting or electrically stimulated single cells were analyzed at 36.0 ± 1.0°C. The imaging of fluo-8 was analyzed for average pixel intensities of regions of interest drawn to include whole cell, following background correction, using an Aquacosmos image-processing system (Hamamatsu Photonics, Hamamatsu, Japan). The hiPSC-CMs were stimulated at 0.5 and 1.0 Hz with 4 ms depolarizing pulses at 20–30 V using platinum electrodes, with an interelectrode distance of 12 mm (Intermedical, Osaka, Japan). The rhythmicity of the spontaneous Ca2+ transient was assessed by calculating the cycle length variability index, defined as the standard deviation of the cycle length/mean cycle length. A solution containing 100 nM isoproterenol (LKT Laboratories, Saint Paul, MN, USA) was prepared fresh before the experiment and applied 5–10 min before data collection. A 10 μM ryanodine solution (Wako Pure Chemical Industries) was added 2–3 min before isoproterenol administration.
The hiPSC-CMs were enzymatically dissociated with collagenase B and Trypsin EDTA and plated onto glass coverslips coated with fibronectin (BD Biosciences). APs were recorded at 36.0 ± 1.0°C in a current clamp mode using a perforated patch-clamp technique. The pipette solution contained 300 μg/ml amphotericin B (Sigma-Aldrich, St Louis, MO, USA) and the following (in mM): 150 KCl, 5 EGTA, 5 MgATP, 10 HEPES, 5 NaCl, 2 CaCl2, pH was adjusted to 7.2 by KOH. The experiments were performed under continuous perfusion of the extracellular solution containing (in mM): 150 NaCl, 5.4 KCl, 1.8 CaCl2, 1 MgCl2, 15 glucose, 15 HEPES, 1 Na-pyruvate (pH adjusted to 7.40 with NaOH) . Patch-clamp pipettes, formed from borosilicate glass with PP-830 (Narishige, Tokyo, Japan) and had a resistance of 4–7 MΩ. APs were recorded from spontaneously contracting and quiescent hiPSC-CMs. All signals were acquired at 10 kHz, digitized with a Digidata 1332A (Axon instruments, CA, USA) and analyzed with a pCLAMP 10.4 software (Axon instruments). Current clamp recordings were performed using a MultiClamp 700B amplifier. Solutions containing 100 nM isoproterenol were applied using a gravitational flow system 5–10 min prior to data collection. We added 1 and 10 μM freshly prepared S107 (Cayman Chemical, Ann Arbor, MI, USA) to the culture medium 2–3 hours prior to the experiments.
Continuous variables are presented as the mean ± SEM. Categorical variables are expressed as frequencies. Differences in the means between two groups were compared using Student’s t-tests. Categorical differences between two groups were evaluated using chi-squared tests. A value of p < 0.05 was considered statistically significant.
We generated hiPSCs from a 37-year-old female patient with CPVT, whose clinical features were previously reported . She first experienced syncope during running or swimming at 15 years of age. Her next episodes of syncope were while riding a bicycle and when under emotional stress caused by a traffic accident at 30 years of age. She then remained free of syncope until the age of 36 years when she experienced an episode upon awakening from a nightmare, at which time she was admitted to the hospital. Polymorphic VT was recorded during an exercise test and an epinephrine provocation test. She was diagnosed with CPVT and began taking carvedilol. However, she had an episode of syncope and polymorphic VT was recorded again during an exercise test despite having received an oral beta-blocker. Subsequently, she underwent implantation of an implantable cardioverter-defibrillator (ICD). At the age of 37, an appropriate ICD discharge occurred and she began taking flecainide, which was effective in preventing polymorphic VT. Genetic analyses identified a missense mutation, c.13759 A>G, p.I4587V, in the RyR2 gene. Her son also had a history of syncope and was a carrier of an identical mutation.
Dermal fibroblasts were obtained from the proband and retrovirally transduced with 4 genes (Oct3/4, Sox2, Klf-4, and c-Myc). As a control, we used an hiPSC line, 201B7, which was similarly generated from a healthy individual . The CPVT-hiPSCs exhibited characteristic human embryonic stem cell morphology and stained positively for pluripotency markers (OCT3/4, SSEA4, TRA1-60) (Fig 1A). The CPVT-hiPSC lines displayed a normal karyotype (Fig 1B). We confirmed the RyR2-I4587V mutation in the CPVT-hiPSCs, but not in the control-hiPSCs (Fig 1C). In order to confirm the pluripotency of generated hiPSCs, we injected hiPSCs into severe combined immunodeficiency (SCID) mice, which led to the formation of teratomas, containing tissue derivatives of three germ layers: pigmented epithelium (ectoderm), gut-like structures (endoderm), and cartilage tissue (mesoderm) (Fig 1D).
Fig 1. Characterization of CPVT-hiPSCs.
(A) CPVT-hiPSC colonies derived from the dermal fibroblasts of a patient with CPVT expressed pluripotency markers, shown by immunostaining. Scale bars = 200 μm. (B) CPVT-hiPSCs maintained the normal karyotype. (C) Sequencing analysis of the RYR2 gene identified the I4587V heterozygous point mutation in the CPVT-hiPSCs. (D) Hematoxylin-eosin staining of teratomas formed from CPVT-hiPSCs showed differentiation of the cells into various tissues derived from all three germ layers: pigmented epithelium (ectoderm), gut-like structures (endoderm), and cartilage tissue (mesoderm). Scale bar = 100 μm.
We differentiated control- and CPVT-hiPSCs into spontaneously contracting cardiomyocytes using an EB differentiation system. Spontaneously beating EBs started to appear at day 7 of differentiation. EBs were plated on fibronectin-coated dishes at day 21 and maintained in the culture medium described above for an additional 2 months. We conducted quantitative real-time PCR in control- and CPVT-hiPSC-CMs at days 30 and 90, and the expression levels of the genes involved in Ca2+ handling are shown in Fig 2. Consistent with previously reported transcriptional profile data on human iPSC/ESC-derived cardiomyocytes , CASQ2 expression levels were extremely low compared to those of adult human cardiomyocytes. However, RyR2 and SERCA2 were already expressed at 1 month similar to adult human myocytes in the control- and CPVT-hiPSC-CMs. Innositol-1,4,5-trisphosphate receptor 2 (IP3R2) and calreticulin were highly expressed in the control- and CPVT-hiPSC-CMs compared to adult human cardiomyocyts (Fig 2). In this study, there was no significant difference in Ca2+ handling gene expression between control- and CPVT-hiPSC-CMs.
Fig 2. Gene expression of calcium handling proteins in hiPSC-CMs.
We performed Ca2+ imaging of spontaneously contracting control- and CPVT-hiPSC-CMs. A considerable number of both control- and CPVT-hiPSC-CMs showed irregular beating rhythms at baseline and after isoproterenol administration (Fig 3A). To evaluate the irregularity parameter, we calculated the cycle length variability index, also known as the coefficient of variation, defined as the cycle length standard deviation divided by the mean of the cycle length. The cycle length variability indices were higher in hiPSC-CMs compared to those in isolated rabbit nodal cells , but there was no significant difference between control and CPVT cells (Fig 3B). In spontaneous beating hiPSC-CMs, we found no significant difference in the frequency of diastolic Ca2+ waves (increased intracellular Ca2+ concentration during the diastolic phase) between control and CPVT cells (Fig 3C).
Fig 3. Ca2+ imaging of spontaneously beating hiPSC-CMs.
(A) Representative tracings of Ca2+ imaging in spontaneously contracting control- and CPVT-hiPSC-CMs. (B) Cycle length variability indices, defined as the standard deviation of the cycle length/mean cycle length of spontaneously beating hiPSC-CMs showed no significant difference between control and CPVT. (C) The percentage of spontaneously contracting control- and CPVT-hiPSC-CMs presenting diastolic Ca2+ waves at baseline and after isoproterenol administration.
Therefore, to minimize the effect of beating intervals, we used electrical field stimulation when recording Ca2+ transients to assess the arrhythmic phenotype in hiPSC-CMs (Fig 4A). At baseline, CPVT-hiPSC-CMs showed diastolic Ca2+ waves significantly more frequently than control-hiPSC-CMs at 0.5 Hz pacing (CPVT: 33%, control: 4%; p < 0.05; Fig 4B). After administration of 100 nM isoproterenol, significantly more frequent diastolic Ca2+ waves were recorded in CPVT-hiPSC-CMs than control-hiPSC-CMs (CPVT: 58%, control: 20% at 0.5 Hz; p < 0.05; CPVT: 43%, control: 12% at 1 Hz; p < 0.05; Fig 4B).
Fig 4. Ca2+ imaging of electrically stimulated hiPSC-CMs.
(A) Representative tracings of Ca2+ imaging in control- and CPVT-hiPSC-CMs with electrical stimulation at 0.5 Hz. Arrows indicate the diastolic Ca2+ waves and arrowheads indicate the triggered activity. Vertical bars indicate the time points that the hiPSC-CMs were stimulated at 0.5 Hz. (B) The percentage of control- and CPVT-hiPSC-CMs presenting diastolic Ca2+ waves at baseline and after isoproterenol administration (at 0.5 and 1 Hz pacing). *p < 0.05.
We treated the CPVT-hiPSC-CMs with ryanodine, a strong ligand of the ryanodine receptor (Fig 5A). With 10 μM ryanodine, the percentage of CPVT-hiPSC-CMs that presented diastolic Ca2+ waves after isoproterenol administration significantly decreased to 9% at 0.5 Hz and 7% at 1 Hz pacing (Fig 5B).
Fig 5. Ryanodine suppressed diastolic Ca2+ waves in CPVT-hiPSC-CMs.
(A) Representative tracings of Ca2+ imaging in CPVT-hiPSC-CMs with (lower) and without (upper) ryanodine. Note diastolic Ca2+ waves (arrows) without ryanodine (upper, right), however, no diastolic Ca2+ waves with ryanodine (lower, right). Vertical bars indicate the time points that the CPVT-hiPSC-CMs were stimulated at 0.5 Hz. (B) Fraction (in %) of CPVT-hiPSC-CMs that showed diastolic Ca2+ waves with and without ryanodine. *p < 0.05.
The percentage of CPVT-hiPSC-CMs that developed DADs during AP recordings did not differ from that of control-hiPSC-CMs under the spontaneously beating condition at baseline and after isoproterenol administration (Fig 6A and 6B). Under regular electrical stimulation, AP recordings revealed no significant difference in the percentage of cardiomyocytes with DADs between control and CPVT at baseline (CPVT: 56%, control: 20% at 1 Hz; CPVT: 38%, control: 14% at 1.5 Hz; Fig 6C and 6D). However, administration of isoproterenol at 100 nM produced significantly more frequent DADs in CPVT-hiPSC-CMs than control-hiPSC-CMs (CPVT: 86%, control: 30% at 1 Hz; p < 0.05; CPVT: 69%, control: 29% at 1.5 Hz; p < 0.05; Fig 6D). We found no early afterdepolarization in the AP recordings of control- and CPVT-hiPSC-CMs when paced at 1 or 1.5 Hz.
Fig 6. AP recordings of hiPSC-CMs.
(A) Representative tracings of AP recordings from spontaneously beating control- and CPVT-hiPSC-CMs. Both control- and CPVT-hiPSC-CMs showed DADs. (B) No significant difference was found in the percentage of spontaneously contracting hiPSC-CMs presenting DADs between control and CPVT. (C) Representative tracings of AP recordings during 1 Hz pacing from control- and CPVT-hiPSC-CMs. CPVT-hiPSC-CMs showed DADs, especially after isoproterenol administration. (D) The percentage of control- and CPVT-hiPSC-CMs that developed DADs at 1 and 1.5 Hz pacing. *p < 0.05.
We treated CPVT-hiPSC-CMs with the 1,4-benzothiazepine derivative S107. Pre-incubation with S107 for 2–3 hours suppressed the frequency of DADs in CPVT-hiPSC-CMs in a concentration-dependent manner (Fig 7A). Incubation with 1 μM S107 significantly decreased the percentage of CPVT-hiPSC-CMs presenting DADs to 33% at 1 Hz pacing after isoproterenol administration. Incubation with 10 μM S107 further decreased the percentage of CPVT-hiPSC-CMs with DADs compared to those lacking S107 (25% at 1 Hz; p < 0.05, 10% at 1.5 Hz; p < 0.05; Fig 7B).
Fig 7. S107 prevented DADs in CPVT-hiPSC-CMs.
(A) Representative tracings of AP recordings from CPVT-hiPSC-CMs following a 2–3 h pre-incubation with 10 μM S107. DADs were not found after isoproterenol application. (B) Fraction (in %) of CPVT-hiPSC-CMs that showed DADs after isoproterenol administration with and without S107 pre-incubation. S107 suppressed DADs in a concentration-dependent manner. *p < 0.05 versus the absence of S107.
Since the recognition of RyR2 as a gene responsible for CPVT , functional analyses have suggested that DADs following Ca2+ leakage from the sarcoplasmic reticulum (SR) are associated with ventricular arrhythmia in CPVT; however, the mechanisms underlying SR Ca2+ leak have not been elucidated. Even now, some patients with CPVT experience syncope or sudden cardiac death. Although we must develop novel therapeutic approaches, it is uncertain whether the results of drug testing in mouse models are replicated in humans. Patient-specific hiPSC-based models of CPVT offer new opportunities for studying drug effects and pathogenetic mechanisms in human cardiomyocytes, however, it is not invariably successful to recapitulate the phenotypes due to the electrophysiological immaturity of hiPSC-CMs. In this study, we precisely evaluated the electrophysiological properties using electrical pacing, and confirmed abnormal diastolic Ca2+ waves during Ca2+ imaging and DADs during AP recordings in CPVT-hiPSC-CMs, which are two major phenotypes shown in CPVT knock-in mouse models [26, 27]. In addition, S107 prevented the development of DADs in CPVT-hiPSC-CMs.
Cardiomyocytes derived from human embryonic stem cells (hESC-CMs) have functional IP3-dependent Ca2+ release and the gene expression level of IP3R2 progressively declines with the maturation of the hESC-CMs . For the first time, we showed that IP3R2 was highly expressed in hiPSC-CMs compared to adult human cardiomyocytes. IP3-dependent Ca2+ signaling has been shown to play an important role during the process of cardiac development. Calreticulin is also an important Ca2+ buffer and a regulator of Ca2+ homeostasis during fetal life. High expression levels of IP3R2 and calreticulin, together with extremely low expression levels of CASQ2, indicate immature Ca2+ handling properties in hiPSC-CMs.
The hiPSC-CMs have irregular beating rhythms resulting from immature electrophysiological properties, which hamper the precise electrophysiological analyses. The cycle length variability indices of spontaneously beating control- and CPVT-hiPSC-CMs were remarkably higher compared to those of rabbit pacemaker cells , indicating highly irregular contraction of hiPSC-CMs. Under spontaneously beating conditions, there were no significant differences in the frequency of diastolic Ca2+ waves or DADs between control- and CPVT-hiPSC-CMs. Ca2+ transients, ion currents (Ica(L), Ito, INaK and INaL), and intracellular ion concentrations (Na+ and K+) in myocytes were reported to vary depending on pacing frequency using in-silico analyses [29, 30]. Therefore, we used electrical stimulation to perform the experiments at equal beating rates and successfully demonstrated significantly more frequent diastolic Ca2+ waves and DADs in CPVT-hiPSC-CMs compared to control cells. Kujala et al reported the presence of early afterdepolarizations in CPVT-hiPSC-CMs ; however, in our experiments, we did not identify any early afterdepolarizations during 1 or 1.5 Hz pacing. The use of 10 μM ryanodine significantly suppressed the diastolic Ca2+ waves of CPVT-hiPSC-CMs. This ligand fully closes ryanodine receptors at micromolar concentrations. Therefore, our results indicate that ryanodine receptors play an important role in the mechanisms of diastolic Ca2+ waves in CPVT-hiPSC-CMs.
Human RyR2 is a large intracellular Ca2+-permeable channel and consists of the N-terminal, central, and C-terminal domains . The C-terminal domain is composed of the I-domain and the transmembrane domain which forms the channel pore. K201 (JTV519), a 1,4-benzothiazepine derivative, was formerly a promising candidate drug for CPVT and was thought to stabilize RyR2 by correcting defective domain-domain interactions in the N-terminal and central domains. K201 prevented the SR Ca2+ leak in failing cardiomyocytes by improving the defective inter-domain interaction between the N-terminal and central domains . However, it failed to present cardioprotective effects on catecholamine-induced DADs and arrhythmia generation in the R4496C+/- mouse model of CPVT with a mutation in the RyR2 transmembrane domain . In addition, K201 was reported to be a multichannel blocker that suppressed ICa(L) and INCX (33 ± 5% reduction in the peak Ica(L) with 0.3 μM K201, the concentration generally used in in vitro assays) . In this context, another 1,4-benzothiazepine derivative, S107, was developed. S107 is one of the Rycals®, small molecules that prevent Ca2+ leakage from the SR by promoting the binding of calstabin or calstabin2 (a channel stabilizing protein) to ryanodine receptor isoform 1 or RyR2. S107 has high specificity for RyR2 and no off-target activity up to 10 μM . In mouse model studies, S107 was effective for preventing ventricular arrhythmias, seizures and atrial fibrillation in CPVT . In the present study, we first confirmed the efficacy of S107 in CPVT-hiPSC-CMs. Three mechanisms have been proposed to underlie leaky RyR2 channels: dissociation of a channel-stabilizing protein (calstabin2) from RyR2 , defective inter-domain folding of RyR2 , and changes in the sensitivity of RyR2 to cytosolic and luminal Ca2+ . The augmented binding of calstabin2 to RyR2 is the only mechanism demonstrated to underlie the effect of S107. Regarding the binding site of calstabin2 on RyR2, the central domain of RyR2 has been reported to interact with calstabin2 . However, Zissimopoulos and colleagues demonstrated that the C-terminal domain (containing the transmembrane domain) of RyR2 interacts with calstabin2 . In our CPVT model, S107 might exert an antiarrhythmic effect by improving binding between calstabin2 and the transmembrane domain of RyR2. S107 can also prevent stress-induced cognitive dysfunction , the progression of heart failure , and muscle weakness in aging  or Duchenne muscular dystrophy . The lead Rycal program is in phase 2 clinical studies for the treatment of heart failure and arrhythmias. These compounds may have great promise for treating patients with CPVT.
There are several limitations in this study. We analyzed CPVT-hiPSC-CMs derived from one RyR2 mutation. It is therefore unclear whether the findings in our model would be confirmed in another CPVT hiPSC-model harboring different mutations in RyR2 or other candidate genes. Other limitations are immature electrophysiological and structural properties of hiPSC-CMs compared to adult CMs, and a fundamental issue that we have developed a cell-based model which did not recapitulate the complex environment of a mammalian heart.
We generated a stem cell-based CPVT model harboring an RyR2 mutation in the transmembrane domain, and it successfully recapitulated the disease phenotype. Electrical pacing was highly useful for analyzing arrhythmogenic features in CPVT-hiPSC-CMs, and the antiarrhythmic effect of S107 was confirmed in this model. This CPVT hiPSC-based model assessed with electrical pacing is a powerful tool that will provide us with a better understanding of the underlying mechanisms and new approaches for screening drugs to establish personalized medicine.
S1 Table. Primers used for sequencing of the human RYR2 gene (exon 94) and qRT-PCR.
We thank Masako Tanaka, Kyoko Yoshida and Aya Umehara for their technical assistance.
Conceptualization: TM Y. Yoshida KS.
Data curation: YW T. Kamakura SN M. Hayano TH Y. Yamamoto HK SH JC.
Funding acquisition: TM T. Kimura.
Methodology: TM KS SM AT.
Project administration: TM Y. Yoshida M. Horie.
Resources: NS MM MK HI.
Supervision: TM Y. Yoshida SO SY M. Horie T. Kimura.
Writing – review & editing: TM Y. Yoshida M. Horie.
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Our thought for today is from Ezekiel 37:14: "I will put my spirit within you, and you shall live."
Let us pray: Living God, breathe into us Your spirit, that these Representatives follow the example of service to the people. Guide them during these last few days as they strive to do what is good for this State. Inspire them to do the responsible thing. Bestow Your blessings upon our Nation, President, State, Governor, Speaker, staff, and all who contribute to the success of this Session. Protect our defenders of freedom as they protect us. Heal the wounds, those seen and those hidden, of our brave warriors who suffer and sacrifice for our freedom. Lord, in Your mercy, hear our prayers. Amen.
Rep. BRADLEY moved that when the House adjourns, it adjourn in memory of Jerry Cook Brown of Hilton Head, which was agreed to.
The House stood in silent prayer for the family of Joseph Robinette "Beau" Biden III.
Whereupon, the Chair appointed Reps. WHITE, PITTS and CLYBURN to the Committee of Conference on the part of the House and a message was ordered sent to the Senate accordingly.
The Senate respectfully invites your Honorable Body to attend in the Senate Chamber today at a mutually convenient time, for the purpose of ratifying Acts.
On motion of Rep. CORLEY the invitation was accepted.
H. 4287 (Word version) -- Reps. Gambrell, Alexander, Allison, Anderson, Anthony, Atwater, Bales, Ballentine, Bamberg, Bannister, Bedingfield, Bernstein, Bingham, Bowers, Bradley, Brannon, G. A. Brown, R. L. Brown, Burns, Chumley, Clary, Clemmons, Clyburn, Cobb-Hunter, Cole, Collins, Corley, H. A. Crawford, Crosby, Daning, Delleney, Dillard, Douglas, Duckworth, Erickson, Felder, Finlay, Forrester, Funderburk, Gagnon, George, Gilliard, Goldfinch, Govan, Hamilton, Hardee, Hart, Hayes, Henderson, Henegan, Herbkersman, Hicks, Hill, Hiott, Hixon, Hodges, Horne, Hosey, Howard, Huggins, Jefferson, Johnson, Jordan, Kennedy, King, Kirby, Knight, Limehouse, Loftis, Long, Lowe, Lucas, Mack, McCoy, McEachern, McKnight, M. S. McLeod, W. J. McLeod, Merrill, Mitchell, D. C. Moss, V. S. Moss, Murphy, Nanney, Neal, Newton, Norman, Norrell, Ott, Parks, Pitts, Pope, Putnam, Quinn, Ridgeway, Riley, Rivers, Robinson-Simpson, Rutherford, Ryhal, Sandifer, Simrill, G. M. Smith, G. R. Smith, J. E. Smith, Sottile, Southard, Spires, Stavrinakis, Stringer, Tallon, Taylor, Thayer, Tinkler, Toole, Weeks, Wells, Whipper, White, Whitmire, Williams, Willis and Yow: A HOUSE RESOLUTION TO RECOGNIZE AND HONOR THE BELTON HONEA PATH HIGH SCHOOL BASEBALL TEAM FOR AN OUTSTANDING SEASON AND TO CONGRATULATE THE PLAYERS ON WINNING THE 2015 CLASS AAA STATE CHAMPIONSHIP TITLE.
H. 4288 (Word version) -- Reps. G. R. Smith, Burns, Chumley, Putnam, Loftis, Hamilton, Nanney, Kennedy, Willis, Bedingfield, Alexander, Allison, Anderson, Anthony, Atwater, Bales, Ballentine, Bamberg, Bannister, Bernstein, Bingham, Bowers, Bradley, Brannon, G. A. Brown, R. L. Brown, Clary, Clemmons, Clyburn, Cobb-Hunter, Cole, Collins, Corley, H. A. Crawford, Crosby, Daning, Delleney, Dillard, Douglas, Duckworth, Erickson, Felder, Finlay, Forrester, Funderburk, Gagnon, Gambrell, George, Gilliard, Goldfinch, Govan, Hardee, Hart, Hayes, Henderson, Henegan, Herbkersman, Hicks, Hill, Hiott, Hixon, Hodges, Horne, Hosey, Howard, Huggins, Jefferson, Johnson, Jordan, King, Kirby, Knight, Limehouse, Long, Lowe, Lucas, Mack, McCoy, McEachern, McKnight, M. S. McLeod, W. J. McLeod, Merrill, Mitchell, D. C. Moss, V. S. Moss, Murphy, Neal, Newton, Norman, Norrell, Ott, Parks, Pitts, Pope, Quinn, Ridgeway, Riley, Rivers, Robinson-Simpson, Rutherford, Ryhal, Sandifer, Simrill, G. M. Smith, J. E. Smith, Sottile, Southard, Spires, Stavrinakis, Stringer, Tallon, Taylor, Thayer, Tinkler, Toole, Weeks, Wells, Whipper, White, Whitmire, Williams and Yow: A HOUSE RESOLUTION TO RECOGNIZE AND HONOR THE PALMETTO FAMILY COUNCIL AND TO CONGRATULATE THE STAFF AND BOARD OF DIRECTORS FOR THEIR SIGNIFICANT IMPACT ON FAMILY VALUES AND RELIGIOUS AND CONSTITUTIONAL FREEDOM IN SOUTH CAROLINA.
H. 4289 (Word version) -- Reps. Willis, Pitts, G. R. Smith, Bedingfield, Alexander, Allison, Anderson, Anthony, Atwater, Bales, Ballentine, Bamberg, Bannister, Bernstein, Bingham, Bowers, Bradley, Brannon, G. A. Brown, R. L. Brown, Burns, Chumley, Clary, Clemmons, Clyburn, Cobb-Hunter, Cole, Collins, Corley, H. A. Crawford, Crosby, Daning, Delleney, Dillard, Douglas, Duckworth, Erickson, Felder, Finlay, Forrester, Funderburk, Gagnon, Gambrell, George, Gilliard, Goldfinch, Govan, Hamilton, Hardee, Hart, Hayes, Henderson, Henegan, Herbkersman, Hicks, Hill, Hiott, Hixon, Hodges, Horne, Hosey, Howard, Huggins, Jefferson, Johnson, Jordan, Kennedy, King, Kirby, Knight, Limehouse, Loftis, Long, Lowe, Lucas, Mack, McCoy, McEachern, McKnight, M. S. McLeod, W. J. McLeod, Merrill, Mitchell, D. C. Moss, V. S. Moss, Murphy, Nanney, Neal, Newton, Norman, Norrell, Ott, Parks, Pope, Putnam, Quinn, Ridgeway, Riley, Rivers, Robinson-Simpson, Rutherford, Ryhal, Sandifer, Simrill, G. M. Smith, J. E. Smith, Sottile, Southard, Spires, Stavrinakis, Stringer, Tallon, Taylor, Thayer, Tinkler, Toole, Weeks, Wells, Whipper, White, Whitmire, Williams and Yow: A HOUSE RESOLUTION TO RECOGNIZE AND HONOR THE SIGNIFICANT SERVICE OF LAURENS ELECTRIC COOPERATIVE AND TO CONGRATULATE THE ORGANIZATION ON ITS SEVENTY-FIFTH ANNIVERSARY OF PROVIDING ELECTRICITY IN THE PALMETTO STATE.
H. 4290 (Word version) -- Reps. Clemmons, Alexander, Allison, Anderson, Anthony, Atwater, Bales, Ballentine, Bamberg, Bannister, Bedingfield, Bernstein, Bingham, Bowers, Bradley, Brannon, G. A. Brown, R. L. Brown, Burns, Chumley, Clary, Clyburn, Cobb-Hunter, Cole, Collins, Corley, H. A. Crawford, Crosby, Daning, Delleney, Dillard, Douglas, Duckworth, Erickson, Felder, Finlay, Forrester, Funderburk, Gagnon, Gambrell, George, Gilliard, Goldfinch, Govan, Hamilton, Hardee, Hart, Hayes, Henderson, Henegan, Herbkersman, Hicks, Hill, Hiott, Hixon, Hodges, Horne, Hosey, Howard, Huggins, Jefferson, Johnson, Jordan, Kennedy, King, Kirby, Knight, Limehouse, Loftis, Long, Lowe, Lucas, Mack, McCoy, McEachern, McKnight, M. S. McLeod, W. J. McLeod, Merrill, Mitchell, D. C. Moss, V. S. Moss, Murphy, Nanney, Neal, Newton, Norman, Norrell, Ott, Parks, Pitts, Pope, Putnam, Quinn, Ridgeway, Riley, Rivers, Robinson-Simpson, Rutherford, Ryhal, Sandifer, Simrill, G. M. Smith, G. R. Smith, J. E. Smith, Sottile, Southard, Spires, Stavrinakis, Stringer, Tallon, Taylor, Thayer, Tinkler, Toole, Weeks, Wells, Whipper, White, Whitmire, Williams, Willis and Yow: A HOUSE RESOLUTION TO HONOR THE MAGNA CARTA WITH ITS UNDERLYING BIBLICAL PRINCIPLES, RECOGNIZING ITS FUNDAMENTAL BASIS FOR WESTERN CIVILIZATION'S LIBERTY UNDER LAW AND ITS ROLE IN THE DEVELOPMENT OF JURISPRUDENCE AND FREEDOM IN OUR NATION AND THROUGHOUT THE WORLD, AND TO DECLARE JUNE 15, 2015, AS "MAGNA CARTA DAY" IN SOUTH CAROLINA.
H. 4291 (Word version) -- Rep. Bannister: A HOUSE RESOLUTION TO AUTHORIZE THE GREENVILLE YOUNG MEN'S CHRISTIAN ASSOCIATION TO USE THE HOUSE CHAMBER AND ANY AVAILABLE COMMITTEE HEARING ROOMS IN THE BLATT BUILDING ON THURSDAY, DECEMBER 3, 2015, AND FRIDAY, DECEMBER 4, 2015, TO CONDUCT THE YOUTH IN GOVERNMENT PROGRAM.
That the members of the South Carolina House of Representatives, by this resolution, authorize the Greenville Young Men's Christian Association to use the House chamber and any available committee hearing rooms in the Blatt Building on Thursday, December 3, 2015, and Friday, December 4, 2015, to conduct the Youth in Government program. If the House is in statewide session, or if the House chamber is otherwise unavailable, the House chamber may not be used on those dates.
Be it further resolved that a copy of this resolution be forwarded to the Executive Director of the YMCA Youth in Government program and to House of Representatives Sergeant at Arms, Mitchell G. Dorman.
H. 4292 (Word version) -- Reps. Erickson, Alexander, Allison, Anderson, Anthony, Atwater, Bales, Ballentine, Bamberg, Bannister, Bedingfield, Bernstein, Bingham, Bowers, Bradley, Brannon, G. A. Brown, R. L. Brown, Burns, Chumley, Clary, Clemmons, Clyburn, Cobb-Hunter, Cole, Collins, Corley, H. A. Crawford, Crosby, Daning, Delleney, Dillard, Douglas, Duckworth, Felder, Finlay, Forrester, Funderburk, Gagnon, Gambrell, George, Gilliard, Goldfinch, Govan, Hamilton, Hardee, Hart, Hayes, Henderson, Henegan, Herbkersman, Hicks, Hill, Hiott, Hixon, Hodges, Horne, Hosey, Howard, Huggins, Jefferson, Johnson, Jordan, Kennedy, King, Kirby, Knight, Limehouse, Loftis, Long, Lowe, Lucas, Mack, McCoy, McEachern, McKnight, M. S. McLeod, W. J. McLeod, Merrill, Mitchell, D. C. Moss, V. S. Moss, Murphy, Nanney, Neal, Newton, Norman, Norrell, Ott, Parks, Pitts, Pope, Putnam, Quinn, Ridgeway, Riley, Rivers, Robinson-Simpson, Rutherford, Ryhal, Sandifer, Simrill, G. M. Smith, G. R. Smith, J. E. Smith, Sottile, Southard, Spires, Stavrinakis, Stringer, Tallon, Taylor, Thayer, Tinkler, Toole, Weeks, Wells, Whipper, White, Whitmire, Williams, Willis and Yow: A HOUSE RESOLUTION TO CELEBRATE THE SIXTIETH ANNUAL BEAUFORT WATER FESTIVAL, TO BE HELD FRIDAY, JULY 17, 2015, THROUGH SUNDAY, JULY 26, 2015, TO ENCOURAGE ALL SOUTH CAROLINIANS TO ATTEND AND ENJOY THIS FAMILY-FRIENDLY EVENT, AND TO WISH ITS ORGANIZERS EVERY SUCCESS.
H. 4294 (Word version) -- Rep. Bingham: A HOUSE RESOLUTION TO RECOGNIZE AND HONOR DR. VENUS J. HOLLAND, SUPERINTENDENT FOR LEXINGTON COUNTY SCHOOL DISTRICT TWO, UPON THE OCCASION OF HER RETIREMENT AFTER A DISTINGUISHED CAREER OF FORTY YEARS IN THE FIELD OF EDUCATION AND TO WISH HER CONTINUED SUCCESS AND HAPPINESS IN ALL HER FUTURE ENDEAVORS.
H. 4300 (Word version) -- Reps. Mitchell, Alexander, Allison, Anderson, Anthony, Atwater, Bales, Ballentine, Bamberg, Bannister, Bedingfield, Bernstein, Bingham, Bowers, Bradley, Brannon, G. A. Brown, R. L. Brown, Burns, Chumley, Clary, Clemmons, Clyburn, Cobb-Hunter, Cole, Collins, Corley, H. A. Crawford, Crosby, Daning, Delleney, Dillard, Douglas, Duckworth, Erickson, Felder, Finlay, Forrester, Funderburk, Gagnon, Gambrell, George, Gilliard, Goldfinch, Govan, Hamilton, Hardee, Hart, Hayes, Henderson, Henegan, Herbkersman, Hicks, Hill, Hiott, Hixon, Hodges, Horne, Hosey, Howard, Huggins, Jefferson, Johnson, Jordan, Kennedy, King, Kirby, Knight, Limehouse, Loftis, Long, Lowe, Lucas, Mack, McCoy, McEachern, McKnight, M. S. McLeod, W. J. McLeod, Merrill, D. C. Moss, V. S. Moss, Murphy, Nanney, Neal, Newton, Norman, Norrell, Ott, Parks, Pitts, Pope, Putnam, Quinn, Ridgeway, Riley, Rivers, Robinson-Simpson, Rutherford, Ryhal, Sandifer, Simrill, G. M. Smith, G. R. Smith, J. E. Smith, Sottile, Southard, Spires, Stavrinakis, Stringer, Tallon, Taylor, Thayer, Tinkler, Toole, Weeks, Wells, Whipper, White, Whitmire, Williams, Willis and Yow: A HOUSE RESOLUTION TO HONOR LEON E. WILES, CHIEF DIVERSITY OFFICER FOR CLEMSON UNIVERSITY, ON THE OCCASION OF HIS RETIREMENT, TO EXTEND DEEP APPRECIATION FOR HIS MANY YEARS OF DISTINGUISHED PUBLIC SERVICE TO THE STATE OF SOUTH CAROLINA, AND TO OFFER BEST WISHES FOR A SATISFYING AND REWARDING RETIREMENT.
H. 4293 (Word version) -- Rep. Quinn: A CONCURRENT RESOLUTION TO REQUEST THAT THE DEPARTMENT OF TRANSPORTATION AND THE DEPARTMENT OF NATURAL RESOURCES RENAME THE "HOPE FERRY LANDING" ON THE SALUDA RIVER THE "J. B. BARKER BOAT LANDING" AND ERECT APPROPRIATE SIGNS OR MARKERS AT THIS LOCATION THAT CONTAIN THIS DESIGNATION.
H. 4295 (Word version) -- Reps. Funderburk, Alexander, Allison, Anderson, Anthony, Atwater, Bales, Ballentine, Bamberg, Bannister, Bedingfield, Bernstein, Bingham, Bowers, Bradley, Brannon, G. A. Brown, R. L. Brown, Burns, Chumley, Clary, Clemmons, Clyburn, Cobb-Hunter, Cole, Collins, Corley, H. A. Crawford, Crosby, Daning, Delleney, Dillard, Douglas, Duckworth, Erickson, Felder, Finlay, Forrester, Gagnon, Gambrell, George, Gilliard, Goldfinch, Govan, Hamilton, Hardee, Hart, Hayes, Henderson, Henegan, Herbkersman, Hicks, Hill, Hiott, Hixon, Hodges, Horne, Hosey, Howard, Huggins, Jefferson, Johnson, Jordan, Kennedy, King, Kirby, Knight, Limehouse, Loftis, Long, Lowe, Lucas, Mack, McCoy, McEachern, McKnight, M. S. McLeod, W. J. McLeod, Merrill, Mitchell, D. C. Moss, V. S. Moss, Murphy, Nanney, Neal, Newton, Norman, Norrell, Ott, Parks, Pitts, Pope, Putnam, Quinn, Ridgeway, Riley, Rivers, Robinson-Simpson, Rutherford, Ryhal, Sandifer, Simrill, G. M. Smith, G. R. Smith, J. E. Smith, Sottile, Southard, Spires, Stavrinakis, Stringer, Tallon, Taylor, Thayer, Tinkler, Toole, Weeks, Wells, Whipper, White, Whitmire, Williams, Willis and Yow: A CONCURRENT RESOLUTION TO RECOGNIZE AND HONOR RICHARD STILTON HAGINS OF KERSHAW COUNTY UPON THE OCCASION OF HIS RETIREMENT AS DIRECTOR OF SALKEHATCHIE-WATEREE CAMP AND TO THANK HIM FOR HIS TWENTY-FIVE YEARS OF SELFLESS SERVICE TO THE PEOPLE OF KERSHAW COUNTY.
H. 4297 (Word version) -- Reps. Govan, Mack, Rutherford, Alexander, Allison, Anderson, Anthony, Atwater, Bales, Ballentine, Bamberg, Bannister, Bedingfield, Bernstein, Bingham, Bowers, Bradley, Brannon, G. A. Brown, R. L. Brown, Burns, Chumley, Clary, Clemmons, Clyburn, Cobb-Hunter, Cole, Collins, Corley, H. A. Crawford, Crosby, Daning, Delleney, Dillard, Douglas, Duckworth, Erickson, Felder, Finlay, Forrester, Funderburk, Gagnon, Gambrell, George, Gilliard, Goldfinch, Hamilton, Hardee, Hart, Hayes, Henderson, Henegan, Herbkersman, Hicks, Hill, Hiott, Hixon, Hodges, Horne, Hosey, Howard, Huggins, Jefferson, Johnson, Jordan, Kennedy, King, Kirby, Knight, Limehouse, Loftis, Long, Lowe, Lucas, McCoy, McEachern, McKnight, M. S. McLeod, W. J. McLeod, Merrill, Mitchell, D. C. Moss, V. S. Moss, Murphy, Nanney, Neal, Newton, Norman, Norrell, Ott, Parks, Pitts, Pope, Putnam, Quinn, Ridgeway, Riley, Rivers, Robinson-Simpson, Ryhal, Sandifer, Simrill, G. M. Smith, G. R. Smith, J. E. Smith, Sottile, Southard, Spires, Stavrinakis, Stringer, Tallon, Taylor, Thayer, Tinkler, Toole, Weeks, Wells, Whipper, White, Whitmire, Williams, Willis and Yow: A CONCURRENT RESOLUTION TO EXPRESS THE PROFOUND SORROW OF THE MEMBERS OF THE SOUTH CAROLINA GENERAL ASSEMBLY UPON THE PASSING OF THE HONORABLE JOSEPH ROBINETTE "BEAU" BIDEN III OF THE STATE OF DELAWARE, AND TO EXTEND THEIR DEEPEST SYMPATHY TO HIS LOVING FAMILY AND HIS MANY FRIENDS AND COLLEAGUES.
H. 4298 (Word version) -- Reps. Putnam and Hill: A JOINT RESOLUTION PROPOSING AN AMENDMENT TO SECTION 7, ARTICLE VI OF THE CONSTITUTION OF SOUTH CAROLINA, 1895, RELATING TO THE CONSTITUTIONAL OFFICERS OF THIS STATE, TO PROVIDE THAT THERE SHALL BE ELECTED BY THE QUALIFIED VOTERS OF THIS STATE A SECRETARY OF TRANSPORTATION WHO SHALL HOLD THIS OFFICE FOR A TERM OF FOUR YEARS, COTERMINOUS WITH THAT OF THE GOVERNOR WHOSE DUTIES AND COMPENSATION MUST BE PRESCRIBED BY LAW.
Rep. PUTNAM asked unanimous consent to have the Joint Resolution placed on the Calendar without reference.
Rep. D. C. MOSS objected.
On motion of Rep. CLARY, with unanimous consent, the Bill was ordered placed on the Calendar without reference.
I came in after the roll call and was present for the Session on Tuesday, June 2.
William "Bill" Hixon              Peter McCoy, Jr.
The SPEAKER PRO TEMPORE granted Rep. YOW a leave of absence for the day due to family medical reasons.
The SPEAKER PRO TEMPORE granted Rep. HORNE a leave of absence for the day due to work obligations.
The SPEAKER PRO TEMPORE granted Rep. MURPHY a temporary leave of absence.
The SPEAKER PRO TEMPORE granted Rep. V. S. MOSS a temporary leave of absence.
Rep. SPIRES moved that the House recede until 3:00 p.m., which was agreed to.
The SPEAKER granted Rep. NEWTON a leave of absence for the remainder of the day due to a family commitment.
The SPEAKER granted Rep. FUNDERBURK a leave of absence for the remainder of the day due to attending an economic development announcement in Kershaw County.
Rep. HAYES asked unanimous consent to recall S. 135 (Word version) from the Committee on Labor, Commerce and Industry.
Rep. CLEMMONS asked unanimous consent to recall S. 667 (Word version) from the Committee on Judiciary.
Rep. J. E. SMITH moved to table the amendment.
I was temporarily out of the Chamber on constituent business during the vote on Amendment No. 1A to H. 3114. If I had been present, I would have voted in favor of the Amendment.
Rep. W. J. MCLEOD made the Point of Order that the Senate Amendments were improperly before the House for consideration since its number and title have not been printed in the House Calendar at least one statewide legislative day prior to such reading.
Rep. TALLON moved to waive Rule 5.10, pursuant to Rule 5.15.
So, the House refused to waive Rule 5.10, pursuant to Rule 5.15.
Rep. RILEY moved that the House recur to the morning hour, which was agreed to.
H. 4301 (Word version) -- Reps. Anderson, Alexander, Allison, Anthony, Atwater, Bales, Ballentine, Bamberg, Bannister, Bedingfield, Bernstein, Bingham, Bowers, Bradley, Brannon, G. A. Brown, R. L. Brown, Burns, Chumley, Clary, Clemmons, Clyburn, Cobb-Hunter, Cole, Collins, Corley, H. A. Crawford, Crosby, Daning, Delleney, Dillard, Douglas, Duckworth, Erickson, Felder, Finlay, Forrester, Funderburk, Gagnon, Gambrell, George, Gilliard, Goldfinch, Govan, Hamilton, Hardee, Hart, Hayes, Henderson, Henegan, Herbkersman, Hicks, Hill, Hiott, Hixon, Hodges, Horne, Hosey, Howard, Huggins, Jefferson, Johnson, Jordan, Kennedy, King, Kirby, Knight, Limehouse, Loftis, Long, Lowe, Lucas, Mack, McCoy, McEachern, McKnight, M. S. McLeod, W. J. McLeod, Merrill, Mitchell, D. C. Moss, V. S. Moss, Murphy, Nanney, Neal, Newton, Norman, Norrell, Ott, Parks, Pitts, Pope, Putnam, Quinn, Ridgeway, Riley, Rivers, Robinson-Simpson, Rutherford, Ryhal, Sandifer, Simrill, G. M. Smith, G. R. Smith, J. E. Smith, Sottile, Southard, Spires, Stavrinakis, Stringer, Tallon, Taylor, Thayer, Tinkler, Toole, Weeks, Wells, Whipper, White, Whitmire, Williams, Willis and Yow: A HOUSE RESOLUTION TO CONGRATULATE CARLETHIA DENTLEY WILLIAMS OF GEORGETOWN COUNTY ON THE OCCASION OF HER NINETY-FIFTH BIRTHDAY AND TO WISH HER A JOYOUS BIRTHDAY CELEBRATION AND MANY YEARS OF CONTINUED HEALTH AND HAPPINESS.
S. 839 (Word version) -- Senators O'Dell and Nicholson: A CONCURRENT RESOLUTION TO RECOGNIZE AND HONOR JUANITA DAVENPORT "NITA" HOZEY, OWNER AND SOLE OPERATOR OF PRAISE UNLIMITED WEDDINGS, LLC, AND TO CONGRATULATE HER FOR BEING NAMED THE 2015 SOUTH CAROLINA WOMEN IN BUSINESS ADVOCATE OF THE YEAR.
S. 843 (Word version) -- Senator Thurmond: A CONCURRENT RESOLUTION TO RECOGNIZE AND COMMEND DR. BRIAN QUATTLEBAUM OF NORTH CHARLESTON FOR HIS SERVICE AS PRESIDENT OF THE SOUTH CAROLINA CHIROPRACTIC ASSOCIATION FOR 2014-2015 AND TO WISH HIM THE BEST IN ALL HIS FUTURE ENDEAVORS.
Rep. QUINN made the Point of Order that the Bill was improperly before the House for consideration since its number and title have not been printed in the House Calendar at least one statewide legislative day prior to second reading.
Rep. LOFTIS asked unanimous consent to recall S. 667 (Word version) from the Committee on Judiciary.
H. 4208 (Word version) -- Reps. Atwater and Long: A BILL TO AMEND THE CODE OF LAWS OF SOUTH CAROLINA, 1976, BY ADDING CHAPTER 30 TO TITLE 27 TO ENACT THE "SOUTH CAROLINA HOMEOWNERS PROTECTION ACT OF 2015" SO AS TO SET FORTH THE MANNER IN WHICH A HOMEOWNERS ASSOCIATION CREATED AFTER 2015 MUST BE ESTABLISHED AND ADMINISTERED, AND TO SET FORTH THE RIGHTS OF THE ASSOCIATION AND ITS OWNERS; TO AMEND SECTION 22-3-10, RELATING TO THE JURISDICTION OF MAGISTRATES, SO AS TO GRANT MAGISTRATES CONCURRENT CIVIL JURISDICTION IN CERTAIN ACTIONS BETWEEN ASSOCIATIONS AND OWNERS; AND TO AMEND SECTION 22-3-20, RELATING TO THE JURISDICTION OF MAGISTRATES, SO AS TO PROVIDE THAT A MAGISTRATE DOES NOT HAVE JURISDICTION OF CERTAIN FORECLOSURE ACTIONS BY AN ASSOCIATION.
Rep. WHIPPER, with unanimous consent, withdrew his request for debate on H. 3008 (Word version); however, other requests for debate remained on the Bill.
Rep. R. L. BROWN, with unanimous consent, withdrew his request for debate on H. 3008 (Word version); however, other requests for debate remained on the Bill.
Rep. HENEGAN, with unanimous consent, withdrew her request for debate on H. 3521 (Word version); however, other requests for debate remained on the Bill.
Rep. TALLON, with unanimous consent, withdrew his request for debate on H. 3008 (Word version); however, other requests for debate remained on the Bill.
Rep. DANING, with unanimous consent, withdrew his request for debate on H. 3008 (Word version); however, other requests for debate remained on the Bill.
Rep. MCEACHERN, with unanimous consent, withdrew his request for debate on H. 3521 (Word version); however, other requests for debate remained on the Bill.
Rep. HILL made the Point of Order that the Senate Amendments were improperly before the House for consideration since its number and title have not been printed in the House Calendar at least one statewide legislative day prior to such reading.
While voting on H. 3583, I intended to vote yea, as I am against any discriminatory boycotts of Israel and all our United States trading partners.
(L) A person who violates the provisions of this section may be prosecuted by the State Grand Jury, pursuant to Section 14-7-1600, when a victim is trafficked in more than one county or a trafficker commits the offense of trafficking in persons in more than one county."
Rep. BRANNON explained the Conference Report.
Rep. D. C. MOSS moved that the House do now adjourn.
The House refused to adjourn by a division vote of 38 to 41.
H. 4185 (Word version) -- Rep. McEachern: A CONCURRENT RESOLUTION TO REQUEST THAT THE DEPARTMENT OF TRANSPORTATION NAME THE INTERCHANGE LOCATED AT THE INTERSECTION OF INTERSTATE HIGHWAY 20 AND UNITED STATES HIGHWAY 321 IN RICHLAND COUNTY "WILLIE R. PORTEE INTERCHANGE" AND ERECT APPROPRIATE MARKERS OR SIGNS AT THIS INTERCHANGE THAT CONTAIN THIS DESIGNATION.
S. 331 (Word version) -- Senator Rankin: A CONCURRENT RESOLUTION TO REQUEST THAT THE DEPARTMENT OF TRANSPORTATION NAME THE PORTION OF US HIGHWAY 17 AT THE INTERSECTION OF FARROW PARKWAY AND SC 707 IN HONOR OF THE BELLAMY FAMILY, AND ERECT APPROPRIATE MARKERS OR SIGNS ALONG THIS PORTION OF HIGHWAY THAT CONTAIN THE WORDS "THE BELLAMY INTERCHANGE".
Rep. HILL moved to recall the following Bill from the Committee on Judiciary.
H. 3716 (Word version) -- Reps. Hill, Putnam, G. R. Smith, Corley, Bedingfield, Chumley, Burns and Loftis: A BILL TO AMEND THE CODE OF LAWS OF SOUTH CAROLINA, 1976, BY ENACTING THE "SOUTH CAROLINA CONSTITUTIONAL CARRY ACT OF 2015"; TO AMEND SECTION 10-11-320, RELATING TO CARRYING OR DISCHARGING OF A FIREARM, SO AS TO DELETE THE TERM "CONCEALABLE WEAPONS' PERMIT" AND REPLACE IT WITH THE TERM "FIREARM"; TO AMEND SECTION 16-23-20, AS AMENDED, RELATING TO THE UNLAWFUL CARRYING OF A HANDGUN, SO AS TO DELETE REFERENCES TO A CONCEALED WEAPONS PERMIT ISSUED TO A PERSON, TO REVISE THE PROVISION THAT ALLOWS A HANDGUN TO BE CARRIED BY A PERSON IN A VEHICLE, AND TO PROVIDE THAT CERTAIN PERSONS WHO ARE AT LEAST TWENTY-ONE YEARS OF AGE MAY CARRY A HANDGUN UNDER CERTAIN CIRCUMSTANCES; TO AMEND SECTIONS 16-23-420 AND 16-23-430, AS AMENDED, BOTH RELATING TO THE POSSESSION OF A FIREARM ON SCHOOL PROPERTY, SO AS TO DELETE REFERENCES TO CONCEALED WEAPON PERMITS, AND TO PROVIDE THAT BOTH SECTIONS DO NOT APPLY TO A PERSON WHO LAWFULLY IS CARRYING A WEAPON; TO AMEND SECTION 16-23-460, AS AMENDED, RELATING TO CARRYING CONCEALED WEAPONS, SO AS TO PROVIDE THAT THIS SECTION DOES NOT APPLY TO HANDGUNS; TO AMEND SECTION 51-3-145, AS AMENDED, RELATING TO CERTAIN ACTS THAT ARE UNLAWFUL IN A STATE PARK, SO AS TO MAKE A TECHNICAL CHANGE; TO AMEND SECTION 23-31-215, AS AMENDED, RELATING TO THE ISSUANCE OF A CONCEALED WEAPON PERMIT, SO AS TO PROVIDE AN EXCEPTION TO THE PROVISION THAT PROHIBITS THE CARRYING OF A CONCEALABLE WEAPON ONTO CERTAIN PREMISES, TO DELETE THE PROVISION THAT PROVIDES FOR THE REVOCATION OF A PERSON'S PERMIT WHEN HE VIOLATES CERTAIN PROVISIONS CONTAINED IN THIS SECTION, AND TO REVISE THE PROVISION THAT ALLOWS CERTAIN PERSONS TO CARRY A CONCEALED WEAPON WITHOUT A PERMIT; TO AMEND SECTIONS 23-31-220 AND 23-31-225, BOTH RELATING TO A PROPERTY OWNER'S RIGHT TO ALLOW A HOLDER OF A CONCEALED WEAPON PERMIT TO CARRY A WEAPON ONTO HIS PROPERTY, SO AS TO MAKE TECHNICAL CHANGES, TO PROVIDE THAT THIS PROVISION REGULATES BOTH PERSONS WHO POSSESS AND DO NOT POSSESS A WEAPONS PERMIT, AND TO DELETE THE PROVISION THAT REQUIRES THE REVOCATION OF A PERMIT FOR A VIOLATION OF CERTAIN PROVISIONS OF LAW, AND TO DELETE A REFERENCE TO THE PROVISION THAT PROVIDES FOR THE ISSUANCE OF CONCEALED WEAPON PERMITS; AND TO AMEND SECTION 23-31-240, RELATING TO PERSONS WHO ARE ALLOWED TO CARRY A CONCEALED WEAPON WHILE ON DUTY, SO AS TO DELETE THE PROVISION THAT REQUIRES THESE PERSONS TO POSSESS A CONCEALED WEAPON PERMIT.
Rep. J. E. SMITH moved to table the motion.
So, the House refused to table the motion to recall H. 3716.
The question recurred to the motion to recall H. 3716 from the Committee on Judiciary.
By voice vote, the House refused to recall H. 3716 from Judiciary.
Rep. WHITE moved to dispense with the balance of the Motion Period, which was agreed to.
Rep. GAGNON moved to adjourn debate on the Bill until Wednesday, June 3, which was agreed to.
Rep. WEEKS moved to adjourn debate on the Bill until Wednesday, June 3, which was agreed to.
/ SECTION 1. The General Assembly finds that it shall be the public policy of this State to protect its citizens from the application of foreign laws, whether secular or religious in nature or origin, when the application of a foreign law will result in the violation of a right guaranteed by the Constitution of this State or of the United States including, but not limited to, due process, freedom of religion, speech, or press, and any right of privacy as specifically defined by the Constitution of this State. The General Assembly fully recognizes the right to contract freely under the laws of this State and also recognizes that this right may be reasonably and rationally circumscribed pursuant to the state's interest to protect and promote rights and privileges granted under the United States or South Carolina Constitution including, but not limited to, due process, freedom of religion, speech, or press, and any right of privacy as specifically defined by the Constitution of the United States or of this State.
"Section 14-1-250. (A) As used in this section, 'foreign law' means any law, legal code, or legal system of a jurisdiction outside of any state or territory of the United States including, but not limited to, international organizations and tribunals regardless of whether such law, legal code, or legal system is actively used or applied by that jurisdiction's courts, administrative bodies, or other formal or informal tribunals.
(B) Any court, arbitration, tribunal, or administrative agency ruling or decision in a matter at issue shall violate the public policy of this State and be void and unenforceable to the extent that any such ruling or decision is based upon any foreign law that would not grant the parties affected by the ruling or decision, at a minimum, the fundamental liberties, rights, and privileges granted under the United States and South Carolina Constitutions including, but not limited to, due process, freedom of religion, speech, or press, and any right of privacy as specifically defined by the Constitution of the United States, or of this State.
(C) A contract or, if severable, a contractual provision which provides for the choice of a foreign law to govern some or all of the disputes between the parties adjudicated by a court of law or by an arbitration panel arising from the contract mutually agreed upon shall violate the public policy of this State and be void and unenforceable entirely or, if severable, to the extent of the violation, if the foreign law chosen would apply any substantive or procedural law that would not grant the parties the same fundamental liberties, rights, and privileges granted under the United States or South Carolina Constitutions including, but not limited to, due process, freedom of religion, speech, or press, and any right of privacy as specifically defined by the Constitution of the United States or of this State.
(D)(1) A contract or, if severable, a contractual provision which provides the courts or arbitration panels in personam jurisdiction over the parties to adjudicate any disputes between parties arising from the contract mutually agreed upon shall violate the public policy of this State and be void and unenforceable entirely or, if severable, to the extent of the violation, if the jurisdiction chosen applies any foreign law to the dispute at issue, that would not grant the parties, at a minimum, the fundamental liberties, rights, and privileges granted under the United States and South Carolina Constitutions including, but not limited to, due process, freedom of religion, speech, or press, and any right of privacy as specifically defined by the Constitution of the United States or the Constitution of this State.
(2) If a resident of this State, subject to personal jurisdiction in this State, seeks to maintain litigation, arbitration, agency, or similarly binding proceedings in this State and if the courts of this State find that granting a claim of forum non conveniens or a related claim would result or likely result in a violation in the foreign forum of the nonclaimant's fundamental liberties, rights, and privileges granted under the United States or South Carolina Constitution with respect to the matter in dispute, then it is the public policy of this State that the claim shall be denied.
(E) This section shall not apply to a church or other religious organization, association, or society, with respect to the individuals of a particular religion regarding matters that are purely ecclesiastical including, but not limited to, matters of calling a pastor, excluding members from a church, electing church officers, matters concerning church bylaws, constitution, and doctrinal regulations and the conduct of other routine church business, when the jurisdiction of the courts of this State would be contrary to the First Amendment of the United States and the Constitution of this State.
Rep. LIMEHOUSE moved to adjourn debate on the Bill until Wednesday, June 3, which was agreed to.
Rep. HODGES moved that the House do now adjourn, which was agreed to.
(R. 80, S. 3 (Word version)) -- Senators L. Martin, Shealy, Malloy, Courson, Fair, Turner, Lourie and Hembree: AN ACT TO AMEND THE CODE OF LAWS OF SOUTH CAROLINA, 1976, SO AS TO ENACT THE "DOMESTIC VIOLENCE REFORM ACT"; TO AMEND SECTION 16-25-10, AS AMENDED, RELATING TO DEFINITIONS FOR PURPOSES OF DOMESTIC VIOLENCE OFFENSES, SO AS TO DEFINE OTHER NECESSARY TERMS; TO AMEND SECTION 16-3-600, AS AMENDED, RELATING TO ASSAULT AND BATTERY OFFENSES, SO AS TO REVISE THE DEFINITION OF "MODERATE BODILY INJURY" TO CONFORM; TO AMEND SECTION 16-25-20, AS AMENDED, RELATING TO DOMESTIC VIOLENCE OFFENSES, SO AS TO RESTRUCTURE THE OFFENSES BY GRADUATING THE PENALTIES INTO DEGREES, DEFINE THE ELEMENTS OF EACH DEGREE, AND PROVIDE A NEW PENALTY STRUCTURE, AMONG OTHER THINGS; TO AMEND SECTION 16-25-65, AS AMENDED, RELATING TO DOMESTIC VIOLENCE OF A HIGH AND AGGRAVATED NATURE, SO AS TO RESTRUCTURE THE OFFENSE, REDEFINE THE ELEMENTS OF THE OFFENSE, AND TO RESTRUCTURE THE PENALTY; TO AMEND SECTION 16-1-60, AS AMENDED, RELATING TO CRIMES DEFINED AS VIOLENT, SO AS TO INCLUDE DOMESTIC VIOLENCE IN THE FIRST DEGREE AS A VIOLENT CRIME; TO AMEND SECTION 17-25-45, AS AMENDED, RELATING TO OFFENSES DEFINED AS "MOST SERIOUS" AND "SERIOUS", SO AS TO ADD THE OFFENSES OF DOMESTIC VIOLENCE OF A HIGH AND AGGRAVATED NATURE AND DOMESTIC VIOLENCE IN THE FIRST DEGREE TO THE LIST OF "SERIOUS" OFFENSES; TO AMEND SECTION 56-7-10, AS AMENDED, RELATING TO UNIFORM TRAFFIC TICKETS, SO AS TO INCLUDE DOMESTIC VIOLENCE IN THE SECOND AND THIRD DEGREE OFFENSES TO THE LIST OF ADDITIONAL OFFENSES FOR WHICH A UNIFORM TRAFFIC TICKET MAY BE ISSUED; TO AMEND SECTION 16-25-30, RELATING TO POSSESSION OF A FIREARM BY A PERSON CONVICTED OF CERTAIN DOMESTIC VIOLENCE OFFENSES, SO AS TO PROVIDE THAT IT IS UNLAWFUL FOR PERSONS CONVICTED OF CERTAIN DOMESTIC VIOLENCE OFFENSES TO SHIP, TRANSPORT, RECEIVE, OR POSSESS A FIREARM OR AMMUNITION UNDER CERTAIN CIRCUMSTANCES AND PROVIDE A TIME FRAME FOR THE RESTORING OF RIGHTS REGARDING SHIPPING, TRANSPORTING, RECEIVING, OR POSSESSING A FIREARM OR AMMUNITION; TO AMEND SECTION 17-15-30 AND SECTION 22-5-510, BOTH AS AMENDED, RELATING TO MATTERS TO BE CONSIDERED WHEN DETERMINING CONDITIONS OF RELEASE ON BOND AND BOND HEARINGS AND INFORMATION TO BE PROVIDED TO THE COURT, RESPECTIVELY, BOTH SO AS TO REQUIRE THE COURT TO CONSIDER IF RELEASE ON BOND WOULD CONSTITUTE AN UNREASONABLE DANGER TO THE COMMUNITY OR AN INDIVIDUAL, TO PROVIDE THAT WHEN A PERSON IS CHARGED WITH A VIOLATION OF CERTAIN DOMESTIC VIOLENCE OFFENSES THAT A BOND HEARING MAY NOT PROCEED WITHOUT THE PERSON'S CRIMINAL RECORD AND INCIDENT REPORT, OR THE PRESENCE OF THE ARRESTING OFFICER, AND TO REQUIRE BOND HEARINGS FOR THESE VIOLATIONS TO BE HELD WITHIN TWENTY-FOUR HOURS AFTER ARREST; TO AMEND SECTION 17-15-10, RELATING TO PERSONS WHO MAY BE RELEASED PENDING TRIAL, SO AS TO REQUIRE THE COURT TO CONSIDER IF RELEASE ON BOND WOULD CONSTITUTE AN UNREASONABLE DANGER TO THE COMMUNITY OR AN INDIVIDUAL; TO AMEND SECTION 16-25-120, AS AMENDED, RELATING TO THE RELEASE OF A PERSON ON BOND WHO IS CHARGED WITH A VIOLENT OFFENSE OR WHEN THE VICTIM IS A HOUSEHOLD MEMBER, SO AS TO PROVIDE THAT THE COURT MUST CONSIDER CERTAIN FACTORS BEFORE RELEASING A PERSON ON BOND; TO AMEND SECTION 17-15-50, RELATING TO AMENDMENT OF AN ORDER RELATING TO BOND, SO AS TO CLARIFY THAT THE COURT WITH JURISDICTION OF THE OFFENSE MAY AMEND THE ORDER AT ANY TIME; TO AMEND SECTION 17-15-55, AS AMENDED, RELATING TO BOND AND THE AUTHORITY OF THE CIRCUIT COURT TO REVOKE BOND UNDER CERTAIN CIRCUMSTANCES, SO AS TO PROVIDE FOR THE PURPOSE OF BOND REVOCATION ONLY THAT A SUMMARY COURT HAS CONCURRENT JURISDICTION WITH THE CIRCUIT COURT FOR TEN DAYS FROM THE DATE BOND IS FIRST SET ON A CHARGE BY THE SUMMARY COURT TO DETERMINE IF BOND SHOULD BE REVOKED; TO AMEND SECTION 16-25-70, AS AMENDED, RELATING TO WARRANTLESS ARREST OR SEARCH FOR A DOMESTIC VIOLENCE OFFENSE, SO AS TO REQUIRE THAT THE MANDATED LAW ENFORCEMENT INVESTIGATION OF A DOMESTIC VIOLENCE OFFENSE MUST BE DOCUMENTED ON AN INCIDENT REPORT FORM WHICH MUST BE MAINTAINED BY THE INVESTIGATING AGENCY; TO AMEND SECTION 16-3-1110, RELATING TO DEFINITIONS FOR PURPOSES OF THE ARTICLE ON COMPENSATION OF VICTIMS OF CRIME, SO AS TO INCLUDE MINOR WITNESSES TO A DOMESTIC VIOLENCE OFFENSE IN THE DEFINITION OF "VICTIM"; TO DIRECT THE DEPARTMENT OF SOCIAL SERVICES IN CONSULTATION WITH THE SOUTH CAROLINA VOUCHER PROGRAM TO PROVIDE CERTAIN CHILDCARE SERVICES TO VICTIMS OF DOMESTIC VIOLENCE TO ENCOURAGE PARTICIPATION IN COURT HEARINGS RELATING TO DOMESTIC VIOLENCE; TO AMEND SECTION 17-22-90, RELATING TO PRETRIAL INTERVENTION PROGRAMS AND AGREEMENTS REQUIRED BY OFFENDERS IN A PROGRAM, SO AS TO REQUIRE THE CIRCUIT SOLICITOR, OR ATTORNEY GENERAL IF APPROPRIATE, TO SELECT AND APPROVE A BATTERER'S TREATMENT PROGRAM FOR USE AS PART OF PRETRIAL INTERVENTION FOR CERTAIN DOMESTIC VIOLENCE OFFENSES; BY ADDING ARTICLE 3 TO CHAPTER 25, TITLE 16 SO AS TO CREATE THE DOMESTIC VIOLENCE ADVISORY COMMITTEE WHOSE PURPOSE IS TO DECREASE THE INCIDENCES OF DOMESTIC VIOLENCE, TO DEFINE NECESSARY TERMS, TO PROVIDE FOR THE MEMBERSHIP OF THE COMMITTEE, TO PROVIDE FOR THE DUTIES OF THE COMMITTEE, AND TO EXEMPT CERTAIN MEETINGS AND INFORMATION FROM THE PROVISIONS OF THE FREEDOM OF INFORMATION ACT AND PROVIDE FOR CONFIDENTIALITY OF CERTAIN INFORMATION RELATED TO THE INVESTIGATION AND REVIEW OF INCIDENCES OF DOMESTIC VIOLENCE BY THE COMMITTEE; BY ADDING ARTICLE 5 TO CHAPTER 25, TITLE 16 SO AS TO RECODIFY THE PROVISIONS OF SECTION 43-1-260, RELATING TO COMMUNITY DOMESTIC VIOLENCE COORDINATING COUNCILS, WITHIN ARTICLE 5; TO AMEND SECTION 59-32-30, AS AMENDED, RELATING TO SUBJECTS TAUGHT IN THE COMPREHENSIVE HEALTH EDUCATION PROGRAM, SO AS TO ADD THE SUBJECT OF DOMESTIC VIOLENCE BEGINNING WITH THE 2016-2017 SCHOOL YEAR; TO REPEAL SECTION 43-1-260 RELATING TO COMMUNITY DOMESTIC VIOLENCE COORDINATING COUNCILS; BY ADDING ARTICLE 18 TO CHAPTER 3, TITLE 16 SO AS TO CREATE PROVISIONS REGARDING PERMANENT RESTRAINING ORDERS, TO DEFINE NECESSARY TERMS, TO PROVIDE PROCEDURES FOR OBTAINING PERMANENT RESTRAINING ORDERS AND EMERGENCY RESTRAINING ORDERS; AND TO AMEND SECTION 22-5-910, AS AMENDED, RELATING TO EXPUNGEMENT OF CRIMINAL RECORDS, SO AS TO INCLUDE FIRST OFFENSE CONVICTIONS FOR DOMESTIC VIOLENCE IN THE THIRD DEGREE IN THE PURVIEW OF THE STATUTE AFTER FIVE YEARS FROM THE DATE OF CONVICTION.
(R. 81, S. 261 (Word version)) -- Senator Thurmond: AN ACT TO AMEND SECTION 59-111-320, CODE OF LAWS OF SOUTH CAROLINA, 1976, RELATING TO PROVISIONS ALLOWING PERSONS AGE SIXTY AND OVER TO ATTEND STATE-SUPPORTED INSTITUTIONS OF HIGHER EDUCATION WITHOUT PAYMENT OF TUITION, SO AS TO REMOVE CRITERIA PROVIDING THESE PERSONS MAY RECEIVE NO COMPENSATION AS FULL-TIME EMPLOYEES.
(R. 82, S. 301 (Word version)) -- Senator Alexander: AN ACT TO AMEND SECTION 40-2-10, AS AMENDED, CODE OF LAWS OF SOUTH CAROLINA, 1976, RELATING TO THE SOUTH CAROLINA BOARD OF ACCOUNTANCY, SO AS TO REVISE THE BOARD'S COMPOSITION; TO AMEND SECTION 40-2-20, RELATING TO DEFINITIONS CONCERNING THE PRACTICE OF ACCOUNTANCY, SO AS TO REVISE CERTAIN DEFINITIONS; TO AMEND SECTION 40-2-30, AS AMENDED, RELATING TO ACTIVITIES REQUIRING LICENSURE OR FIRM REGISTRATION, SO AS TO REPLACE A REFERENCE TO THE TERM "FINANCIAL STATEMENTS" WITH THE WORD "INFORMATION" AND TO ADD AN APPROPRIATE CROSS-REFERENCE; TO AMEND SECTION 40-2-35, RELATING TO CERTIFIED PUBLIC ACCOUNTANT LICENSURE REQUIREMENTS, SO AS TO REQUIRE APPLICANTS TO UNDERGO CERTAIN STATE AND FEDERAL CRIMINAL RECORDS CHECKS, TO REQUIRE CONTINUING EDUCATION OR ADDITIONAL EXPERIENCE, AS APPLICABLE, FOR APPLICANTS WHO DELAY SUBMITTING AN APPLICATION FOR A SUBSTANTIAL PERIOD OF TIME AFTER PASSING THE CERTIFIED PUBLIC ACCOUNTING EXAMINATION OR OBTAINING ACCOUNTING EXPERIENCE, AND TO PROVIDE A NECESSARY DEFINITION; TO AMEND SECTION 40-2-40, RELATING TO QUALIFICATIONS FOR REGISTRATION OF CERTIFIED PUBLIC ACCOUNTING FIRMS, SO AS TO PROVIDE THAT A SIMPLE MAJORITY OF THE FIRM OWNERSHIP MUST BE CERTIFIED PUBLIC ACCOUNTANTS, TO PROVIDE QUALIFICATIONS AND CONTINUING PROFESSIONAL EDUCATION REQUIREMENTS FOR NONCERTIFIED PUBLIC ACCOUNTANT FIRM OWNERS, TO DELETE PROHIBITIONS AGAINST OWNERSHIP BY INVESTORS AND COMMERCIAL ENTERPRISES, AND TO GIVE THE BOARD OF ACCOUNTANCY THE DISCRETION TO CHARGE RELATED FEES; TO AMEND SECTION 40-2-80, AS AMENDED, RELATING TO THE INVESTIGATION OF COMPLAINTS AND DISCIPLINARY PROCEEDINGS, SO AS TO PROVIDE THE DEPARTMENT OF LABOR, LICENSING AND REGULATION MAY REQUIRE STATE AND FEDERAL CRIMINAL RECORDS CHECKS IN CONDUCTING SUCH INVESTIGATIONS AND PROCEEDINGS, TO PROHIBIT USE OF CERTAIN CRIMINAL CHARGE DISMISSALS OR RESTITUTION PAYMENTS AS EVIDENCE OF MISCONDUCT SUBJECT TO DISCIPLINE, AND TO PROVIDE FOR THE RECOVERY OF RELATED COSTS; TO AMEND SECTION 40-2-250, AS AMENDED, RELATING TO LICENSE RENEWALS, TO PROVIDE THAT RENEWAL APPLICATIONS MUST BE FILED ON OR BEFORE FEBRUARY FIRST, TO GIVE THE BOARD DISCRETION TO CHARGE RELATED FEES, AND TO PROVIDE THAT LATE FILINGS MAY RESULT IN LAPSE, REINSTATEMENT FEES, AND SANCTIONS; TO AMEND SECTION 40-2-255, RELATING TO REGISTRATION RENEWALS, SO AS TO PROVIDE THAT RENEWAL APPLICATIONS MUST BE FILED ON OR BEFORE FEBRUARY FIRST, TO GIVE THE BOARD DISCRETION TO CHARGE RELATED FEES, AND TO PROVIDE THAT LATE FILINGS MAY RESULT IN LAPSE AND SANCTIONS; AND TO AMEND SECTION 40-2-560, RELATING TO ISSUANCE OF LICENSES, SO AS TO ADD A CROSS-REFERENCE.
(R. 83, S. 437 (Word version)) -- Senators Campsen, Reese, Gregory, Hutto, Cleary, Campbell, Cromer, Young, Bryant, Williams, Bennett, Johnson, Hembree, O'Dell, Davis, Fair, Hayes and Verdin: AN ACT TO AMEND THE CODE OF LAWS OF SOUTH CAROLINA, 1976, BY ADDING SECTION 59-29-240 SO AS TO ENACT THE "JAMES B. EDWARDS CIVICS EDUCATION INITIATIVE", TO DEFINE THE TERM "CIVICS TEST", TO REQUIRE ALL STUDENTS OF PUBLIC OR CHARTER SCHOOLS IN THIS STATE TO TAKE THE CIVICS TEST PRODUCED BY THE UNITED STATES CITIZENSHIP AND IMMIGRATION SERVICES AS PART OF THE UNITED STATES GOVERNMENT REQUIRED CREDIT, TO ALLOW SCHOOL DISTRICTS TO RECOGNIZE STUDENTS WHO RECEIVE A PASSING GRADE ON THE TEST, TO DIRECT THE RESPECTIVE SCHOOLS TO REPORT RESULTS TO THE SOUTH CAROLINA EDUCATION OVERSIGHT COMMITTEE FOR INCLUSION IN THE REPORT CARD FOR EACH SCHOOL, TO PROVIDE NO SCHOOL OR SCHOOL DISTRICT MAY CHARGE A FEE FOR THE TEST, AND TO PROVIDE THAT THE REQUIREMENTS OF THIS SECTION APPLY TO ANY STUDENT ENTERING THE NINTH GRADE BEGINNING WITH THE 2016-2017 SCHOOL YEAR.
(R. 84, S. 592 (Word version)) -- Senator Campsen: AN ACT TO AMEND SECTION 50-11-710, AS AMENDED, CODE OF LAWS OF SOUTH CAROLINA, 1976, RELATING TO THE HUNTING OF FERAL HOGS, COYOTES, AND ARMADILLOS AT NIGHT, SO AS TO RESTRUCTURE THE EXISTING PROVISIONS THAT REGULATE THE HUNTING OF THESE ANIMALS, AND TO PROVIDE FOR THE NIGHT HUNTING OF THESE ANIMALS BY A PERSON WITH A CENTER FIRE RIFLE USING CENTER FIRE AMMUNITION OR SUBSONIC CENTER FIRE AMMUNITION.
(R. 85, H. 3083 (Word version)) -- Reps. Huggins, Kennedy, Clary, Corley, Weeks, Whipper and Gilliard: AN ACT TO AMEND THE CODE OF LAWS OF SOUTH CAROLINA, 1976, SO AS TO ENACT THE "SOUTH CAROLINA OVERDOSE PREVENTION ACT" BY ADDING CHAPTER 130 TO TITLE 44 SO AS TO ALLOW CERTAIN MEDICAL PROFESSIONALS TO PRESCRIBE OPIOID ANTIDOTES FOR INDIVIDUALS WHO THE MEDICAL PROFESSIONAL BELIEVES IN GOOD FAITH ARE AT RISK OF EXPERIENCING AN OPIOID OVERDOSE, TO REQUIRE MEDICAL PROFESSIONALS TO PROVIDE INSTRUCTIONAL INFORMATION TO A PERSON TO WHOM THE MEDICAL PROFESSIONAL PRESCRIBES AN OPIOID ANTIDOTE, TO ALLOW PHARMACISTS TO DISPENSE OPIOID ANTIDOTES PURSUANT TO A PRESCRIPTION, TO ALLOW CAREGIVERS AND FIRST RESPONDERS TO ADMINISTER OPIOID ANTIDOTES TO INDIVIDUALS WHO THE CAREGIVER OR FIRST RESPONDER BELIEVES IN GOOD FAITH ARE AT RISK OF EXPERIENCING AN OPIOID OVERDOSE, TO ALLOW PRESCRIBERS TO PRESCRIBE STANDING ORDERS FOR OPIOID ANTIDOTES TO FIRST RESPONDERS AND FOR FIRST RESPONDERS TO POSSESS THESE OPIOID ANTIDOTES, AND TO PROVIDE PROTECTIONS FROM CIVIL AND CRIMINAL LIABILITY FOR PRESCRIBING, DISPENSING, OR ADMINISTERING OPIOID ANTIDOTES, AND FOR OTHER PURPOSES.
(R. 86, H. 3264 (Word version)) -- Rep. Taylor: AN ACT TO AMEND THE CODE OF LAWS OF SOUTH CAROLINA, 1976, BY ADDING ARTICLE 137 TO CHAPTER 3, TITLE 56 SO AS TO PROVIDE FOR THE ISSUANCE OF "AMERICAN RED CROSS SPECIAL LICENSE PLATES".
(R. 87, H. 3880 (Word version)) -- Reps. Ott, Hixon, Pitts, Riley and Johnson: AN ACT TO AMEND SECTION 50-11-20, AS AMENDED, CODE OF LAWS OF SOUTH CAROLINA, 1976, RELATING TO CERTAIN TERMS AND THEIR DEFINITIONS THAT RELATE TO THE MIGRATORY WATERFOWL COMMITTEE, THE CREATION OF THE COMMITTEE, ITS MEMBERSHIP, AND RESPONSIBILITIES, SO AS TO INCREASE ITS MEMBERSHIP BY ONE WHO SHALL BE A DESIGNEE OF DELTA WATERFOWL OF SOUTH CAROLINA WHO IS NOT A PAID EMPLOYEE.
(R. 88, H. 3888 (Word version)) -- Reps. Cole, Allison, Brannon, Chumley, Forrester, Hicks, Mitchell and Tallon: AN ACT TO AMEND SECTION 7-7-490, AS AMENDED, CODE OF LAWS OF SOUTH CAROLINA, 1976, RELATING TO THE DESIGNATION OF VOTING PRECINCTS IN SPARTANBURG COUNTY, SO AS TO CONSOLIDATE AND RENAME CERTAIN PRECINCTS, AND TO REDESIGNATE THE MAP NUMBER ON WHICH THE NAMES OF THESE PRECINCTS MAY BE FOUND AND MAINTAINED BY THE REVENUE AND FISCAL AFFAIRS OFFICE.
(R. 89, H. 4166 (Word version)) -- Reps. Pitts and Willis: AN ACT TO AMEND ACT 779 OF 1988, AS AMENDED, RELATING TO LAURENS COUNTY SCHOOL DISTRICTS 55 AND 56, SO AS TO REAPPORTION THE SEVEN SINGLE-MEMBER ELECTION DISTRICTS FROM WHICH MEMBERS OF LAURENS COUNTY SCHOOL DISTRICT 56 MUST BE ELECTED, TO DESIGNATE A MAP NUMBER ON WHICH THESE SINGLE-MEMBER ELECTION DISTRICTS ARE DELINEATED, AND TO PROVIDE DEMOGRAPHIC INFORMATION PERTAINING TO THE REAPPORTIONED ELECTION DISTRICTS.
H. 4086 (Word version) -- Reps. Allison, Alexander, Anderson, Anthony, Atwater, Bales, Ballentine, Bamberg, Bannister, Bedingfield, Bernstein, Bingham, Bowers, Bradley, Brannon, G. A. Brown, R. L. Brown, Burns, Chumley, Clary, Clemmons, Clyburn, Cobb-Hunter, Cole, Collins, Corley, H. A. Crawford, Crosby, Daning, Delleney, Dillard, Douglas, Duckworth, Erickson, Felder, Finlay, Forrester, Funderburk, Gagnon, Gambrell, George, Gilliard, Goldfinch, Govan, Hamilton, Hardee, Hardwick, Hart, Hayes, Henderson, Henegan, Herbkersman, Hicks, Hill, Hiott, Hixon, Hodges, Horne, Hosey, Howard, Huggins, Jefferson, Johnson, Jordan, Kennedy, King, Kirby, Knight, Limehouse, Loftis, Long, Lowe, Lucas, Mack, McCoy, McEachern, McKnight, M. S. McLeod, W. J. McLeod, Merrill, Mitchell, D. C. Moss, V. S. Moss, Murphy, Nanney, Neal, Newton, Norman, Norrell, Ott, Parks, Pitts, Pope, Putnam, Quinn, Ridgeway, Riley, Rivers, Robinson-Simpson, Rutherford, Ryhal, Sandifer, Simrill, G. M. Smith, G. R. Smith, J. E. Smith, Sottile, Southard, Spires, Stavrinakis, Stringer, Tallon, Taylor, Thayer, Tinkler, Toole, Weeks, Wells, Whipper, White, Whitmire, Williams, Willis and Yow: A CONCURRENT RESOLUTION TO PROMOTE UNDERSTANDING OF THE DESTRUCTIVE EFFECTS OF IRON DISORDERS ON THE HEALTH OF OUR CITIZENS, TO ENCOURAGE MEDICAL RESEARCH AND EDUCATION TO STEM THE TIDE OF THESE DISORDERS, AND TO DECLARE THE MONTH OF JULY 2015 AS "IRON DISORDERS AWARENESS MONTH" IN THE STATE OF SOUTH CAROLINA.
At 5:13 p.m. the House, in accordance with the motion of Rep. BRADLEY, adjourned in memory of Jerry Cook Brown of Hilton Head, to meet at 10:00 a.m. tomorrow.