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models via a Bayesian Network approach Finally we are collaborating with National Marine Fisheries Service NMFS and WDFW to coordinate ROV methods and data analyses to ensure that our products are tuned to the management needs of coastal Washington Intended Products By combining community data with existing acoustic imagery of benthic habitat our project will 1 generate habitat suitability models for kelp forest species including species of management concern such as sunflower sea stars and bull kelp Nereocystis luetkeana We will 2 make our field and analytical methods open source so that other researchers can utilize and expand upon them and this will result in 3 peer reviewed publications and conference talks Additionally our work will 4 provide a playbook for how to integrate ROV technology and AI algorithms into marine protected area evaluation and kelp forest monitoring programs Finally once these methods are established 5 in collaboration with Olympic Coast National Marine Sanctuary OCNMS NMFS Makah Tribe and WDFW the Seattle Aquarium intends to initiate a long term research program around Neah Bay and along the Olympic Coast thus the methods we develop here will help springboard a broader vision of coastal research and monitoring Conservation is a core

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text:
tenet of the Seattle Aquarium s mission The Seattle Aquarium s mission is inspiring conservation of our marine environment Our conservation programs focus on moving toward a future that embodies climate resilience sustainable seas and clean waters We envision a future in which ocean and coastal communities survive and thrive where we can meet the needs of the present without compromising the communities or ecosystems of tomorrow Our conservation work encompasses four pillars research and field conservation animal care and rehabilitation policy and advocacy and social change The work we propose here is a direct reflection of these values and by teaming up with PSRF WDFW NMFS and OCNMS the outreach education and research capacity of the Seattle Aquarium will be aimed at addressing a conservation need obtaining benthic species abundance and distribution data across a larger spatial extent that will directly further conservation and management objectives of our state federal and non profit partners Networking No single individual or entity alone can address the complex challenges facing coastal systems in a changing climate and this proposal represents our desire to work together with state federal and non profit entities responsible for coastal management data collection and conservation in Washington

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text:
State We are collaborating with WDFW and NMFS to coordinate our efforts with entities already engaged in ROV work in Washington and we have partnered with PSRF so that our ROV surveys take place along existing long term subtidal monitoring sites The Seattle Aquarium will regularly host partner meetings which through partnership with OCNMS will also draw upon the expertise of members from existing regulatory and oversight entities within Washington State such as the Intergovernmental Policy Council the OCNMS Sanctuary Advisory Council and the Washington Coastal Marine Advisory Committee Budget and Justification i Personnel 40 000 00 to launch a post doctoral fellowship position for Zachary Randell at the Seattle Aquarium covering 45 of his salary and benefits for one year Randell will lead field and analytical efforts as well as communication amongst collaborators ii Travel 10 000 00 for vessel time gas etc to access dive and ROV sites in Puget Sound ii Equipment N A Blue Robotics Blue2 ROV obtained via Sea Otter Foundation and Trust grant iv Supplies N A v Total Matching Funds Acoustic ROV Tracking System provided by WDFW 25 000 Seattle Aquarium match 10 time Erin Meyer 20 time Shawn Larson 25 time Amy

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text:
Olsen total 50 000 vi Total Requested Amount 50 000 00 Remotely Operated Vehicles ROVs Artificial Intelligence AI methods of image analysis and benthic transect methods within bull kelp forests Zachary Randell Megan Williams Shawn Larson A central goal of sea otter conservation is the preservation or restoration of the ecological functioning sea otters provide along coastal ecosystems Challenging the evaluation of this functioning however is our limited ability to survey benthic community structure ROVs have the potential to expand the spatial extent across which we collect data and make inferences in kelp forests but thus far ROVs have yet to be systematically integrated into long term kelp forest monitoring programs As part of emerging Seattle Aquarium research we have customized a ROV to operate within and conduct video surveys within kelp forests all to better understand how sea otters along the Olympic Peninsula affect kelp forest community structure and resilience Good morning General Have you ever tried to find Geoduck siphons underwater That s the exact task facing biologists with Tulalip Tribes Natural Resources and is one typically achieved by SCUBA surveys However the Tulalip biologists were keen to explore whether ROV Nereo was up to the task and

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text:
yesterday Megan and I went out on a Tulalip research vessel and flew ROV Nereo just offshore of Kingston The tide was ripping and the visibility was poor all the warm sunny weather has kicked up the first of the season s algae blooms but ROV Nereo performed admirably See a short video thank you Megan for the quick turnaround editing this Can you find the Geoduck siphons Look for the pairs of black holes the black being the shadow created by the relatively little light that penetrates down into the siphons as the ROV flies over an example Geoduck individual is outlined in red and attached here To give you a sense of the current ROV Nereo battled note 0 59 and 1 15 in the video linked above these are two points at which we let off of the ROV s thrusters entirely and you see the vehicle instantly pushed back by the current before we re engage forward motion And to give you a sense of how murky it was check out recorded video from the ROV s 1080p built in camera Thanks to the placement of our downward facing lights our camera settings and video editing

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text:
from Megan the quality of our video intended for data collection e g the GoPro video linked in the 2nd paragraph is MUCH better than what one might expect given the poor visibility Lessons learned The Geoduck siphons are most visible underneath the ROV thus they d likely want to shoot in the linear GoPro camera setting relative to the wide view mode we use The Tulalip would likely want to fly closer to the substrate than we do probably about 0 8m instead of the 1 0m we typically target Finally they d want to target slack tide to make flying the vehicle easier unlike what we did yesterday In short it was a great first day working with the Tulalip and we look forward to further supporting them if they indeed decide to obtain and customize a BlueROV2 similar to ROV Nereo AI GOES BELOW THE SURFACE USING TECHNOLOGY TO MAKE A DIFFERENCE FOR LOCAL KELP Kelp forests are critical for ecosystem health They provide food and habitat for marine species of all kinds including endangered salmon and southern resident orcas They also sequester carbon mitigating the effects of ocean acidification Check out to learn more about the

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importance of kelp forests in the Salish Sea Bull kelp forms the trees in these underwater forests It attaches to hard surfaces on the seafloor and grows with incredible speed up to 2 feet per day reaching lengths of up to 100 feet in a single season But that s if it s growing Once abundant throughout the Salish Sea kelp forests have declined drastically over the past several decades with a reported 60 overall loss of bull kelp in our local waters since 1980 and up to 95 in some areas What s puzzling researchers though is that kelp forests to thrive in parts of the Salish Sea including sections of Elliott Bay along Seattle s urban waterfront and directly below the Aquarium s pier PARTNERING WITH THE PORT OF SEATTLE TO LEARN MORE Now to study kelp forests in Elliott Bay using a remotely operated vehicle or ROV which we ve fondly named Nereo short for Nereocystis luetkeana the scientific name for bull kelp Research of this kind has typically been undertaken with scientific scuba divers doing surveys below the surface or satellite imagery captured from far above Both methods have their drawbacks while divers can observe many

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species along the seafloor they re limited by the amount of gas they can carry on their backs and ultimately can t cover much ground Satellites of course can cover much larger areas but aren t able to capture kelp that hasn t reached the water s surface or other species that live below it Enter the ROV The compact ROV that is Historically these machines have been large bulky and expensive and deployed from large vessels to explore deep water which isn t what s needed to study kelp forests in relatively shallow depths ROV Nereo from Blue Robotics is small relatively inexpensive customizable and maneuverable a critically important feature when navigating long ropes of kelp and floating fronds With cameras facing forward and downward lights and sensor equipment it can capture images of more species over a larger area than a scientific diver Nereo is easy to deploy and pilot and equipped with sonar so it will stay at a consistent altitude above seafloor as well as GPS to track real time positioning All that and it s operated with an Xbox controller KELP CONSERVATION MEETS ARTIFICIAL INTELLIGENCE Since the summer of 2022 led by the Aquarium s

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text:
Research Scientist Dr Zachary Randell we ve been using Nereo for benthic or seafloor surveys at eight sites in Elliott Bay And here s where things get even more futuristic we re harnessing the power of to interpret the still images and video being captured by Nereo s cameras Open source AI algorithms can be taught to review images and identify what s in them at a speed that simply wouldn t be possible for a human being or even a team of humans Using Nereo and AI we can learn about kelp abundance and distribution in other words the number of kelp plants and where they are in Elliott Bay see surfaces it s growing or not growing on and discover which animal species are present competing with kelp eating it or simply living within it With this information in hand we ll better understand why bull kelp is growing in some places but not others and learn what conditions are needed for healthy abundant kelp forests That knowledge can help inform habitat restoration efforts underway throughout the region by our partners at the and others The ultimate goal To reverse declines of local kelp forests in Elliott Bay

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text:
and throughout Washington waters and develop solutions to rebuild the essential habitat that they provide WORK We were recently thrilled to learn that Dr Randell and team have been awarded a grant by the their work on bull kelp conservation and restoration Kelp forest complexity connectivity and resilience with and without keystone species along the Olympic Coast Principal Investigator PI Shawn Larson1 Co PIs Ken Collins1 Blake Feist2 William Jasper3 Zachary Randell1 Collaborators Henry Carson4 Casey Clark4 Nicole Harris5 Darcie Larson1 Dayv Lowry2 Bob Pacunski4 Jonathan Scordino3 Jamey Selleck2 8 Jodie Toft6 Jenny Waddell5 Teal Waterstrat7 Seattle Aquarium1 NOAA National Marine Fisheries Service NMFS 2 Makah Tribe3 Washington Department of Fish and Wildlife WDFW 4 Olympic Coast National Marine Sanctuary OCNMS 5 Puget Sound Restoration Fund PSRF 6 United States Fish and Wildlife Service USFWS 7 Natural Resources Consultants NRC 8 Rationale 2000 character limit including spaces This project will investigate kelp forest community complexity connectivity and resilience in the western Strait of Juan de Fuca SJDF and northern region of Olympic Coast National Marine Sanctuary OCNMS Managing benthic species such as kelp invertebrates and reef fish require abundance and distribution data yet the logistical demands of data collection have

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text:
precluded landscape scale surveys of kelp forests and instead spatially limited information is gathered by SCUBA divers and from recreational and commercial landings Remotely operated underwater vehicles ROVs can capture data over larger geographic areas but their large size and expense have restricted ROV usage to exploring deep locations unsuitable for divers However recent technological advances have resulted in ROVs that are small affordable deployable from small vessels and able to capture high resolution imagery The work proposed here will leverage the respective strengths of ROVs SCUBA divers and visual observations of sea otters a keystone predator to initiate a new type of long term program to research and monitor Coastal Ecosystem Health a WSG critical program area ROVs will capture kelp forest community structure and associated flora and fauna across a larger geographic extent than previously achievable divers will measure kelp invertebrate and reef fish recruitment and researchers will observe sea otter abundance distribution and foraging behavior We will use machine learning methods with ROV imagery to classify species abundances and percent coverage and we will pair these data with habitat characteristics to create habitat suitability models for kelp invertebrates reef fish and sea otters By placing subtidal sites

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text:
along a gradient of sea otter density we will evaluate the extent to which sea otters modify benthic community structure species diversity and kelp forest resilience particularly following pulse perturbation events associated with climate change Objectives 2000 character limit including spaces Objective 1 1 1 To the best of our knowledge ROV protocols do not exist for kelp forest surveillance at scale thus we will first test and establish protocols for consistently capturing high resolution still images and video of the benthos via a small maneuverable and customizable ROV 1 2 We have partnered with NMFS and the WDFW to develop ROV protocols and conduct overlapping surveys via ROV imagery diver imagery and diver observations This 1 1 overlap of methods will investigate how images processed via machine learning contrast to observational data Objective 2 2 1 Deploy an initial network of long term decade subtidal monitoring sites with five offshore of Neah Bay in the SJDF and five along the northwestern portion of the Olympic Coast ROVs surveys will obtain benthic imagery 2 2 Diver surveys will measure kelp sea urchin and reef fish recruitment Objective 3 3 1 Adapt existing machine learning technologies to classify species abundances and

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