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integration of ROV operations into existing and future Makah coastal research All code and analyses including machine learning algorithms will be shared with the Tribe It is also our intent that this ROV and associated statistical analyses be available to other questions of interest For example the Makah invited us to partner with them and the WDFW on a proposal to the Bureau of Indian Affairs BIA This proposal would fund three projects one of which plans to use drop cameras to monitor the existence and extent of urchin barrens around Neah Bay If this BIA proposal is successful and if the WSG work proposed here proceeds the Seattle Aquarium will provide machine learning algorithms to calculate sea urchin abundances from these images We could expand this BIA project by incorporating ROVs to obtain additional urchin barren imagery We also propose to directly support existing and future Makah personnel and operations Specifically we have budgeted to provide part time support to the Marine Mammal Biologist who will assist with our sea otter foraging and distribution surveys We have also budgeted to provide part time support for a seasonal technician who will collect marine mammal observations participate in ROV operations in

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text:
the field and receive training on machine learning analyses 5 2 Outreach and engagement with coastal communities PI Larson and co PI Randell will spend significant time on the coast and in the field for this project and we will maximize this time by 1 engaging with coastal schools and community activities 2 mentoring student interns and 3 bringing the Seattle Aquarium s Teacher Professional Development program to school districts along the Outer Coast Specifically PI Larson and co PI Randell will present at Cape Flattery School District schools regarding our project and some of the ecological challenges facing coastal ecosystems We are also collaborating with Nicole Harris OCNMS Education Specialist and we will integrate our outreach and engagement into her ongoing work For example certain aspects of our methods align well with the Marine Advanced Technology Education MATE This program provides the opportunity for teams of middle and high school students to develop test and compete ROVs in regional and national competitions In order to connect these students to how our ROV monitors their coast we will bring our ROV to this competition and we will develop a product demonstration challenge replicating the coastal work including a mock kelp

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forest with e g urchin tests and abalone shells that student s ROVs could gather images of in the pool We also propose to mentor students with the Makah Fisheries Management MFM Summer Internship Program In collaboration with Jonathan Scordino we will take interns into the field to experience ROV surveys and we will expose them to computer science via machine learning analyses The MFM program culminates with students preparing scientific posters and presenting research to family friends elders and other community members at the Makah Senior Center We propose to expand this communication experience by having MFM interns present their research to Seattle Aquarium volunteers and staff as part of the existing Lunch Learn communication program This early exposure to field work computer science and research communication will provide valuable skills and experiences which interns can subsequently leverage in future applications and personal statements Finally we have teamed up with Darcie Larson Senior Manager of Community Engagement Inclusion at the Seattle Aquarium to bring the Seattle Aquarium s Teacher Professional Development TPD program to teachers from Neah Bay Clallam Bay Forks Quileute Tribal School Lake Quinault and Taholah Numerous TPD modules have been developed and delivered by the Seattle

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text:
Aquarium Topics envisioned for this project include existing modules such as marine science and biology climate change education sea otter research and STEM integration In Year 1 we have budgeted to develop new modules such as the ecological status of Washington s coastal ecosystems and how researchers are using emerging technologies and analytical approaches such as ROVs and machine learning respectively to tackle anthropogenic and climate challenges along the coast Also in Year 1 PI Larson and co PI Randell will present our ideas for classroom engagement at a 2022 workshop organized by Nicole Harris at OCNMS Year 2 the Seattle Aquarium will host a workshop that presents these TPD modules to teachers from the above school districts and schools and Sarah Brenkert the Principal Evaluator at the Seattle Aquarium will evaluate the efficacy of TPD module delivery 5 3 Biannual meetings to coordinate project objectives methods and products No single individual or entity alone can address the challenges facing coastal systems in a changing climate and this proposal represents our desire to work with tribal state federal and non profit entities responsible for data collection conservation and management within coastal Washington In step with WSG s cross cutting principal

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text:
of Partnerships we have partnered or are collaborating with end users who will translate our products into conservation and management actions including representatives from Makah Tribe OCNMS NMFS WDFW PSRF and USFWS We propose to gather our Co PIs partners and collaborators and coastal management leaders together for biannual meetings at the Seattle Aquarium in Years 1 2 These meetings will ensure project objectives field and analytical methods and products are all focused upon activities most relevant to coastal management and conservation Biannual meetings will occur in the spring and fall at the Seattle Aquarium And with networking assistance from Jenny Waddell at OCNMS we will also invite representatives from the multiple entities already established to oversee coastal species and habitat within OCNMS and the broader state of Washington including the Intergovernmental Policy Counsel the Sanctuary Advisory Council and the Washington Coastal Marine Advisory Committee Co PI Randell commits to engage with and make presentations to these existing oversight entities on a regular on ongoing basis 5 4 Management implications for imperiled coastal species One of the central challenges to managing benthic species is the limitation of currently available data ROVs have the potential to radically alter the spatial resolution

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text:
and extent to which we survey the benthos but they have yet to be systematically integrated into kelp forest monitoring programs Our tests of ROV survey parameters and the implementation of ROV kelp forest surveys at scale and our scrutiny of the taxonomic resolution attainable from machine learning classification of ROV imagery will directly contribute to the advancement of kelp forest surveillance analysis and evaluation And once sustained as a long term monitoring program this WSG project will provide data on community structure recruitment and ecological resilience all part of WSG s Healthy Coastal Ecosystems critical program area for decades to come Within Washington our project will directly contribute to the conservation and management of the following taxa Reef fish In 2003 the Pacific Fishery Management Council delivered its environmental impact statement suggesting that the Northeast Pacific bottomfish fishery was in danger of being overfished Pacific Fishery Management Council 2003 We are collaborating with NMFS and WDFW to ensure our ROV surveys data and habitat suitability modeling further the objectives in the rockfish recovery plan Specifically rockfish abundances extracted from ROV images and diver surveys will assist stock assessments and will regularly contribute to Status Review Updates Kelp distribution in

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text:
Puget Sound has experienced a marked decline in recent decades Palmer McGee 2019 This is partly due to poor water quality influenced by pollution altered nutrient dynamics Khangaonkar et al 2018 and sediment deposition Our work will directly further several objectives of the Puget Sound Kelp Conservation and Recovery Plan Calloway et al 2020 e g our data will describe kelp distribution and trends and our habitat suitability modeling will identify optimal habitat for ongoing bull kelp restoration efforts The pinto abalone population is at critically low levels in Washington and faces potential collapse Excessive harvest by recreational fishers and substantial poaching were the main drivers of pinto abalone decline Bouma 2007 and while the recreational fishery has been closed since 1994 the population has not recovered From 1992 to 2017 pinto abalone counts declined 97 percent at index stations which when combined with lack of evidence for natural reproduction Bouma et al 2012 Rothaus et al 2008 led WDFW to list pinto abalone as a state endangered species in 2019 Pinto abalone recovery is one of PSRF s They are collaborating with the WDFW Seattle Aquarium and others to breed rear and outplant pinto abalone at numerous locations within

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text:
Puget Sound We are collaborating with Jodie Toft at PSRF to align field and analytical efforts For example the abundance of pinto abalone along the Olympic Coast and the western SJDF remains almost entirely unknown due to the lack of fine scale and widespread monitoring programs Our diver surveys will enable a more rigorous assessment of abundances and if present the habitat characteristics these abalone associate with will provide valuable additional information to help guide PSRF s and their partners outplanting efforts Sunflower sea stars experienced a 90 6 global population decline and are now listed as critically endangered by the IUCN after the global disease outbreak of Sea Star Wasting Syndrome SSWS that started in 2013 2014 Gravem et al 2020 Harvell et al 2004 Benthic surveys suggest that sunflower sea star loss in locations that lack other sea urchin predators may lead to increased sea urchin abundances and an increased impact of sea urchin consumption upon benthic kelp and invertebrate species Burt et al 2018 Seattle Aquarium unpublished data The abundance and distribution of sunflower sea stars along the Olympic Coast remains largely unknown and our work will help determine the extent to which this species survived or

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text:
is rebounding Furthermore a collaboration is underway to hatchery raise and outplant sunflower sea stars and just as with pinto abalone the habitat suitability models stemming from our work if sunflower sea stars are found will guide sunflower sea star restoration efforts Alom et al 2019 Sea Otters our entire study is designed to investigate the extent to which sea otters modify the trajectory of kelp forest dynamics particularly following pulse perturbations such as anomalous warm water or marine disease emergency events associated with climate change Given that sea otters once spanned the entirety of the Northeast Pacific Coast i e from the Aleutian Archipelago to Baja California our findings are relevant not only to Washington but to the broader Northeast Pacific coast For example both Oregon and northern California are evaluating the prospect of either directly facilitating a sea otter translocation Oregon or of using captive reared and rehabilitated sea otter pups to help expand the sea otter range beyond the current biogeographical barriers north of Santa Cruz and east of Santa Barbara California These efforts are being directed not by a desire to recover sea otters themselves but rather to restore coastal functioning services and ecological health that

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text:
sea otters provide Data Sharing Plan Title Kelp forest complexity connectivity and resilience with and without keystone species along the Olympic Coast Lead PI Dr Shawn Larson Contact Information Curator of Conservation Research Seattle Aquarium 1484 Alaskan Way Seattle Washington 98101 206 618 3762 Dataset Description Products derived from this project will consist of the following Imagery ROV and diver video and still images and associated metadata Primary data Kelp and sea urchin recruitment abundances from ecosystem experiments and YOY rockfish recruitment abundances from seasonal SMURF arrays Sea otter location abundance and foraging behavior including prey type size dive time and surface time Derived data Descriptive metadata meta analyses database s and analysis scripts associated with each objective Derived data from AI classification of the nearshore marine benthos imagery include abundances and percent coverage estimates of benthic invertebrates macroalgae and rockfish Species diversity will be calculated from abundance and percent coverage data via indices such as Shannon s Diversity Simpson s Diversity and Simpson s Evenness Metadata and collection All imagery products and data will be associated with specific geocoordinates latitude longitude date surveyed and other relevant metadata dependent upon the imagery platform e g ROV diver survey type e

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text:
g swath transect SMURF Imagery products primary data and derived data will be collected by project partners according to the procedures described in the project narrative Sample Data and Script Storage Data will be recorded electronically and on underwater slates for the subset of information that scientific SCUBA divers collect in situ Data recorded in the field will then be transferred to a database program where it can be exported in a variety of formats for use in various statistical software web based data and metadata portals and publications Original underwater paper data collection sheets will be scanned and archived as searchable PDF files Data and source citation for the meta analyses will be collated and stored in a relational database Copies of all electronic files e g csv data files txt metadata files pdf field notes and datasheet files and the database and original photo and video files and associated metadata will be stored on the Seattle Aquarium s network servers and backed up through its three tiered archival system with backups every four hours Cohesity weekly Azure Cool and quarterly AWS Glacier Storage of annotated scripts e g R Python Mathematica will make use of a Git repository

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text:
for version control with final versions also stored and backed up through the Seattle Aquarium s archival system Folder and filenames will be sufficiently descriptive to quickly and uniquely identify their contents Dissemination Methods Final quality controlled metadata will be submitted to the Data Observation Network for Earth DataONE for long term storage and dissemination Additionally a stated objective of the Northwest Association of Networked Ocean Observing Systems NANOOS is to expand into hosting modeling data graphs and maps we will also work with our colleagues at NANOOS to explore whether or not our derived data products and or imagery are of interest to their team We will coordinate with OCNMS and other state and federal partners to identify additional portals that are appropriate or preferable Assessments of species distributions and habitat use will be collected for up to 6 months after the previous year s field season The data will be available to the public upon request starting June 2023 one year after the first full field season in 2022 The primary contact for data access and metadata will be Co PI Randell z randell seattleaquarium org We plan to submit our results to peer reviewed scientific journals and

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