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quality monitoring stations information plugged into models regarding habitat suitability for new kelp growth For example the algorithm may indicate that habitat is particularly friendly to kelp based on known variables such as substrate and species present If kelp doesn t succeed researchers know another variable is influencing survival For example salmon hatch in rivers then come into Puget Sound to dine on the eggs of Pacific herring which spawn in kelp beds After fattening up on the caviar salmon migrate to open seas Without the eggs salmon face reduced survival rates A lack of salmon can lead to a depletion of healthy orca populations which feed on salmon The film revealed surprises too such as a remarkable abundance of kelp crabs which munch on kelp throughout its growing season Could these crabs be hampering the kelp s growth It s too early to tell yet so the project will continue its investigations this summer Other contributing factors may include the seafloor foundation kelp thrive n or environmental variables water temperatures kelp spore placement and movement ocean currents or small scale water flow differences such as water movement between two piers Remotely Operated Vehicles ROVs and artificial intelligence AI along

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text:
the Olympic Coast By Dr Zachary Randell Seattle Aquarium One of the central challenges to understanding the health and population status of kelp invertebrates and fishes along the seafloor is the ability to collect data on abundance how many individuals are present and distribution where individuals are Typically gathering such data involves scientific SCUBA divers though there are limitations to this method In particular SCUBA divers can only cover so much seafloor given the extreme logistical challenges associated with operating in an inhospitable environment ROVs have the potential to greatly expand our ability to collect data along the seafloor especially in remote and rugged locations where SCUBA divers may not have easy or frequent access However thus far ROVs have historically been large bulky expensive and are typically operated off of large vessels Such ROVs are not typically suited for coastal relatively shallow kelp forest operations However recent advancements in ROV technology have produced models that are small customizable and relatively inexpensive The BlueROV2 by Blue Robotics1 is one such ROV In partnership with Makah Fisheries Management Cape Flattery School District Quileute Natural Resources the Quileute Tribal School and Olympic Coast National Marine Sanctuary OCNMS we have customized a BlueROV2

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text:
dubbed ROV Nereo after the scientific name for Bull kelp Nereocystis luetkeana and brought it out to the Olympic Coast ROV Nereo has two primary missions 1 conduct surveys within canopy forming bull kelp forests and 2 engage with coastal schools and communities To advance the first objective we teamed up with OCNMS to fly ROV Nereo above the transects survey locations that OCNMS and NOAA National Marine Fisheries Service SCUBA divers survey along the Olympic Coast annually in the summer You can see some of the downward facing video the ROV gathered off of Tatoosh Island at the link below2 To further the second objective outreach and engagement we conducted several events to share ROV Nereo and its mission along the Olympic Coast In partnership with Cape Flattery School District we worked with Bill Paco Monett and Camden Jones to fly ROV Nereo as part of a Schools Without Walls event In the Makah Marina we flew the ROV along a sunken vessel and students were able to experience the flight in real time via an outdoor television display You can see some of that footage at the link below3 Later in the summer we teamed up with Makah

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text:
Fisheries Management MFM and Jonathan Scordino and Liz Allyn both with MFM were kind enough to take us out on their vessel along with three MFM Interns We once more flew the ROV this time in kelp forests and along urchin barrens and students were able to experience the ROV flight first hand from the surface Further south ROV Nereo made another school appearance in Alice Ryan s classes at the Quileute Tribal School in La Push where students learned about kelp forest ecology subtidal monitoring and how ROVs and AI can help us tackle some of the challenges facing coastal ecosystems and communities in a changing climate Additionally Jennifer Hagan with Quileute Natural Resources was kind enough to provide vessel support so that we could fly ROV Nereo during the OCNMS subtidal surveys whereupon we tried to survey off of Cape Johnson and Tatoosh Island with ROV Nereo Unfortunately a massive algal bloom rendered visibility a mere 1 2 feet which we found insufficient for ROV surveys This was a humbling lesson about the limitations of even cutting edge technology and the need to choose ROV survey windows carefully to align with periods where the water is relatively clear

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text:
Stepping back and in terms of data collection the broader purpose of our ROV surveys is to deepen our understanding of the patterns of ecological resilience of kelp invertebrates and fish species throughout coastal Washington particularly in the wake of changing climate conditions This means conducting long term monitoring where we come back year after year to survey at specific locations We are at the early stages of this though we have made great strides in the past year customizing a ROV and developing kelp forest survey protocols Finally another core purpose of our work is to enable other entities to develop similar ROV programs Towards that end we are making as much of our research open source as possible we have several GitHub repositories6 where we have made ROV videos photos media events project synopses and certain funding proposals public It is our hope that this material can help others who may be interested in starting a similar ROV program The more eyes and cameras we have on the seafloor the more we will be able to understand the status and health of coastal ecosystems in a changing climate 1 2 3 4 5 6 Seven students from Neah

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text:
Bay School observe the outdoor display which transmits the real time camera feed from ROV Nereo Three Makah Fisheries Management Interns and Zachary Randell prepare to fly ROV Nereo east of Neah Bay ROV Nereo a Blue Robotics BlueROV2 customized to conduct kelp forest surveys ROV Nereo alongside Megan Williams Research Technician at the Seattle Aquarium with the full topside command console everything necessary to operate the ROV Monitoring Puget Sound kelp forests with unmanned systems and artificial intelligence Puget Sound Partnership Monitoring to Accelerate Recovery Category 1 Monitoring assessment and reporting of the status and trends of Vital Sign indicators Project start date November 1st 2021 Project end date November 1st 2022 Principal Investigator PI Shawn Larson Curator of Conservation Research Seattle Aquarium Co Investigators Zachary Randell1 Blake Feist 2 Bob Pacunski3 Jodie Toft4 Seattle Aquarium1 National Marine Fisheries Service2 Washington Department of Fish and Wildlife3 Puget Sound Restoration Fund4 Abstract Kelp forests in Puget Sound provide habitat and refuge to numerous culturally important species and those of significant management concern including state federally and internationally listed threatened and endangered species such as pinto abalone Haliotis kamtschatkana sunflower sea stars Pycnopodia helianthoides yelloweye and bocaccio rockfish Sebastes ruberrimus and

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text:
Sebastes paucispinis respectively and several stocks of salmon Understanding the distribution functioning and overall health of Puget Sound s coastal kelp forests is vital for supporting regional management and conservation of coastal resources particularly within the context of changing ocean conditions such as increasing water temperature A central challenge to coastal management and conservation is the inability to survey the seafloor across a broad spatial extent Scientific SCUBA divers are readily able to survey relatively small areas on the order of 10 100s of meters but as kelp forests exhibit high spatial variability and rich spatio temporal dynamics inference from a few locations cannot easily be generalized out to broader scales And while aerial and satellite imagery provide powerful insight into the patterns of canopy forming kelp species that reach the sea surface these images are unable to capture turf or understory algae benthic invertebrates or fish Remotely operated vehicles ROVs have the potential to capture benthic data across a comparatively larger spatial extent but thus far their size and expense has mostly restricted ROV usage to exploring deep locations unsuitable for divers However technological advancement has produced ROV models that are small affordable customizable capture high resolution imagery and

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text:
are deployable from small vessels In conjunction with state federal and non profit partners we propose to bring the next generation of monitoring technology including unmanned systems and artificial intelligence AI algorithms into the kelp forests of Puget Sound Specifically we propose to develop standardized survey protocols to obtain high resolution video of the benthos via a small maneuverable ROV Given the volume of data we intend to collect it would be impractical for human observers to annotate all the imagery Instead we will harness advancements in AI specifically machine learning methods such as supervised classification to 1 identify individual objects such as sea urchins sea stars rockfish and certain algae and 2 obtain percent coverage estimates for colonial and or sessile taxa where individuals are not easily distinguished such as encrusting and articulated coralline algae red and brown understory algae and invertebrates such as sponges cup corals hydroids tunicates and bryozoans Alignment with Cross cutting principals climate change and priority information needs Kelp distribution in Puget Sound has experienced a marked decline in recent decades This is partly due to poor water quality that is influenced by pollution altered nutrient dynamics and sediment deposition Other challenges to kelp growth

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text:
include the invasive seaweed Sargassum muticum along with increasing water temperatures associated with global climate change Yet kelp growth sequesters carbon and locally reduce acidity thus the conservation and restoration of kelp and other coastal macrophytes such as seagrasses now comprise Blue Carbon strategies for offsetting carbon emissions Given that kelp exhibit high rates of primary production and provide robust ecological functioning via habitat and food for numerous coastal species the recovery or lack thereof of kelp in Puget Sound will significantly impact the broader nearshore ecosystem Emerging unmanned systems such as small maneuverable and relatively inexpensive ROVs are a step forward in our capacity to obtain information about the benthos thus the development of kelp forest ROV survey protocols will directly contribute to the monitoring of existing and future Puget Sound Vital Signs and their indicators Specifically our project will further the objectives of New Indicators by expanding the capacity to capture abundance and biomass of Groundfish Benthic Invertebrates as part of the Thriving Species and Food Web Goal Furthermore our ROV methods will simultaneously capture information regarding Potential Future Indicators via imagery of understory kelp abundance and condition part of the Beaches and Marine Vegetation Vital Sign Project

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text:
Approach The Seattle Aquarium was recently awarded a grant to purchase the a small customizable ROV On the underside of this ROV we will stage two image stabilized cameras light strobes and laser size guides to estimate size and biomass Next we will scrutinize the efficacy ROV operations in a variety of environmental conditions that characterize kelp forests In conjunction with Puget Sound Restoration Fund PSRF and in partnership with NOAA National Marine Fisheries Service NMFS we will conduct ROV surveys along PSRF s long term subtidal index sites in Puget Sound This will allow us to explicitly contrast how taxonomic resolution varies between ROV obtained images processed via machine learning versus diver collected data and we will also determine if the ROV imagery captures cryptic taxa such as pinto abalone And via a ROV tracking system from Washington Dept of Fish and Wildlife WDFW our processed imagery will include geospatial coordinates that will enable our data to be combined with habitat layers to generate habitat suitability models via a Bayesian Network approach Expected outcomes and articulated use of project outputs By combining community data with existing benthic side scan sonar data our project will 1 generate habitat suitability models

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for kelp forest species including species of management concern such as sunflower sea stars bull kelp Nereocystis luetkeana listed and unlisted species of rockfish Sebastes spp juvenile salmonids and other bottomfish often associated with rocky reefs and kelp We will 2 make our field and analytical methods open source so that other researchers can utilize and expand upon them and this will result in 3 peer reviewed publications and conference talks The machine learning analytical pipeline we develop will be generalized such other researchers studying an unrelated ecosystem could adapt the code train the algorithm and analyze their own images Additionally our work will 4 provide a playbook for how to integrate ROV technology and AI algorithms into marine protected area evaluation and kelp forest monitoring programs enabling concordance with historical and ongoing monitoring efforts Engagement Plan No single individual or entity alone can address the complex challenges facing coastal systems in a changing climate and this proposal represents our desire to work together with state federal and non profit entities responsible for coastal management data collection and conservation in Washington State We have partnered with WDFW and NMFS to coordinate the development and implementation of ROV protocols and we

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text:
have partnered with PSRF so that our ROV surveys take place along existing long term subtidal monitoring sites We will structure our research around the Puget Sound Kelp Forest Conservation and Recovery Plan and WDFWs Puget Sound Rockfish Conservation Plan Our research will directly support these plans by obtaining bottomfish and kelp distribution abundance and temporal trend data Furthermore we are currently collaborating with PSRF and WDFW to recover endangered pinto abalone in Washington we will be rearing pinto abalone annually starting in June 2021 for grow out and release into the San Juan Islands By collaborating with entities already engaged in ROV and diver subtidal monitoring our work will leverage these efforts to coordinate the development and implementation of novel survey methods and in doing so we will maximize our collective ability to monitor and manage coastal species in Puget Sound Conservation is a core tenet of the Seattle Aquarium s mission The Seattle Aquarium s mission is inspiring conservation of our marine environment Our conservation programs focus on moving toward a future that embodies climate resilience sustainable seas and clean waters We envision a future in which all ocean and coastal communities survive and thrive where we can

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