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about the awarded grant The Sea Otter Foundation Trust will provide any report forms and other necessary materials for written follow up reporting regarding funding awards In addition to the above I accept that if my the work proposed herein changes in any way I or named organization must inform the Sea Otter Foundation Trust regarding the change or changes as soon as possible and understand that the Sea Otter Foundation Trust reserves the right to revoke the funding grant for a full refund to Sea Otter Foundation Trust if the scope of work changes inexorably from the work scope stated within this application or accompanying reports CEO Executive Director Advising Professor Date Higher Education or Other Project Applicant Date The Sea Otter Foundation Trust SOFT looks forward to working with you and or your organization to ensure the survival and recovery of sea otters in their habitats This Sea Otter Foundation and Trust grant provided the first seed funding to start the process of initiating a new nearshore climate resilience project at the Seattle Aquarium With this funding we purchased the Blue2 ROV as described above This ROV will allow us to test and develop protocols for obtaining video

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text:
and photographic imagery of the benthos And via machine learning methods we will process imagery to obtain abundances for conspicuous species such as sea urchins sea stars certain sponges certain macrophytes and fishes and percent cover estimates of colonial and or aggregated species such as sponges hydroids tunicates bryozoans understory red algae and brown algae Together these abundance and percent coverage estimates when obtained 1 across a large spatial extent and 2 in areas with varying sea otter density will provide key information about how sea otters and environmental variables affect kelp forest community structure and ecosystem resilience along the Washington Coast Our most immediate objective is to test the ROV in the field We have scheduled a series of days when Seattle Aquarium personnel are in the field operating out of Sekiu August 16th 20th to test the ROV We will obtain benthic imagery along areas that Seattle Aquarium divers are concurrently conducting rockfish surveys allowing us to test ROV operations in the field and the concordance between data derived from ROV imagery and diver surveys Aside from purchasing the ROV and scheduling preliminary field trials our accomplishments the past 6 months include 1 submitting multiple grants to obtain

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text:
additional funding for this project 2 engaging with partners and collaborators on funding proposals and ROV methods development 3 training initial proof of concept machine learning algorithms to classify abundances and percent coverages of benthic species such as purple urchins 1 We have submitted multiple grants to obtain the full funding necessary to initiate this project These include a 1 9 million over four years proposal to NOAA NOS NCCOS a Washington Sea Grant and Letters of Intent LOI to Puget Sound Partnerships and the SeaDoc Society We received Very High and Excellent reviews on our NCCOS proposal but ultimately it was declined because our proposal did not thoroughly address population connectivity which was an important component of the RFP Furthermore our PSP and SeaDoc Society LOIs were also declined though our project once again was not ideally suited to the specific objectives of these funders Our Washington Sea Grant proposal is pending given the large cohort of partners and collaborators that joined us on this proposal we are tentatively optimistic and we will find out the verdict in September 2 In the process of putting together funding proposals we have engaged with numerous state federal tribal and non profit

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entities operating in Washington These include the Makah Olympic Coast National Marine Sanctuary OCNMS NOAA National Marine Fisheries Service NMFS Washington Dept of Fish and Wildlife WDFW Puget Sound Restoration Fund PSRF Natural Resources Consultants NRC and the US Fish and Wildlife Service USFWS These entities have all expressed excitement and encouragement regarding the development of our project and they have all joined us either as partners or collaborators on our proposals In particular we are working with Bob Pacunski at WDFW Blake Feist at NOAA NMFS and Dayv Lowry at NRC all of whom have extensive ROV experience to develop ROV protocols that compliment ongoing ROV research 3 In order to establish proof of concept we have trained and applied supervised classification to photos of the benthos to 1 obtain abundances of purple urchins and 2 obtain percent coverage estimates of purple urchins The abundance estimates were derived from Azure software and the percent coverage estimates utilized CoralNet s open source algorithms These initial applications of machine learning provided insight such as the importance of obtaining imagery at a consistent scale e g consistently 1m above the benthos and angle Our next steps here include applying CoralNet algorithms to

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text:
the preliminary imagery we will collect in August with the Blue2 ROV Remotely Operated Vehicles ROVs and Artificial Intelligence AI methods of analysis to inform kelp forest conservation and restoration in the Salish Sea Hope Spot Background Kelp particularly canopy forming kelp is critical to a healthy Salish Sea Kelp provides essential habitat and nursery grounds for a diversity of species from marine mammals and birds to forage fish rockfish and salmon to crabs sea stars and abalone These ecosystems also provide coastal resilience through buffering the nearshore to the effects from climate change Unfortunately Puget Sound within the Salish Sea has experienced widespread kelp declines in recent decades with an estimated 65 loss of the canopy forming bull kelp Nereocystis leutkeana since 1980 This is partly due to poor water quality that is influenced by pollution altered nutrient dynamics sediment deposition and increasing water temperatures Despite these regional kelp losses in Puget Sound bull kelp now consistently grows in certain locations such as parts of Elliott Bay including the Elliott Bay Marina Centennial Park and around Piers 59 and 60 managed by the Seattle Aquarium These are some of the most developed and trafficked locations in all of Puget

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text:
Sound yet bull kelp is not only present but is thriving Bull kelp restoration via twine seeded with kelp spores is a primary tool to help recover kelp populations and the ecological functioning kelp forests provide However bull kelp restoration methods in the Salish Sea are at a relatively early stage and we do not fully understand the substrate types environmental conditions and broader community factors that associate with persistent bull kelp forests For example the Port of Seattle the Port and Puget Sound Restoration Fund PSRF teamed up to conduct kelp restoration in Elliott Bay these efforts succeeded in growing adult bull kelp populations in certain locations but failed in others the precise reasons behind the respective successes and failures are unknown One of the central challenges to evaluating kelp forest health is the limitation of currently available data regarding kelp abundance and distribution along the seafloor Scientific SCUBA divers can gather high resolution taxonomic information but the spatial extent that they survey is logistically limited Aerial satellite imagery provides powerful global scale insight for kelp species that reach the sea surface but no information about understory kelp invertebrates or fishes living along the seafloor Remotely operated vehicles ROVs

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text:
have the potential to gather ecologically important subtidal data while significantly expanding the spatial extent across which we survey kelp forests but ROVs have yet to be systematically incorporated into long term kelp forest monitoring programs Integrate our novel survey methods to help guide bull kelp conservation and restoration In partnership between the Seattle Aquarium and the Port we customized a small ROV to conduct benthic surveys within canopy kelp forests Dubbed ROV Nereo this ROV a BlueROV2 from Blue Robotics is small lightweight relatively inexpensive and maneuverable It is rated to a depth of 100m has a 150m tether and has reliably surveyed while pushing against a 3kt current out along the Olympic Peninsula It has proven more than capable of handling the nearshore subtidal conditions throughout coastal Washington In an effort to make information as accessible as possible we have made public code ROV videos documentation hardware etc here In order to further bull kelp conservation and restoration in the Salish Sea Hope Spot we propose to more formally integrate our ROV surveys and AI methods of image analysis into ongoing and future kelp conservation and restoration efforts The ROV obtains orders of magnitude more data that SCUBA

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text:
divers alone can collect and this will help us identify what types of substrate kelp requires what other species positively and negatively associate with bull kelp and the environmental conditions bull kelp requires We specifically envision Mission Blue could help us advance the following three domains _1_ Expand our partnership with the Port by integrating our ROV into current and future conservation and restoration projects In additional to our scheduled ROV surveys we propose to use the ROV to conduct surveys before during and after the deployment of octo pots octopus habitat structures the Port is pursuing to help recover the ecological functioning octopuses provide in the Salish Sea by consuming kelp crabs a species believed to limit bull kelp via herbivory Likewise we propose to conduct our ROV surveys before during and after the Port and PSRF deploy their bull kelp seeded twine to better understand the factors affecting restoration efficacy _2_ Use species and substrate information derived from our AI methods of image analysis to develop species habitat suitability models that will guide bull kelp conservation Our use of open source AI methods of image analysis will generate the large amount of data necessary to construct species habitat

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text:
suitability models Because our ROV surveys are spatially explicit i e we track the ROV s position underwater via an acoustic GPS system we can overlay our data onto existing larger scale layers such as bathymetry and seafloor type This will enable us to calculate for example the probability of encountering bull kelp given the precise underlying substrate type and species present These models will significantly advance our ability to guide future kelp forest conservation and restoration efforts _3_ Work with local entities to develop their own ROV AI programs One of the core motivations for us is to develop low cost monitoring technology and open source analytical capabilities that other entities can adopt Towards that end we are working with multiple entities in Washington to help them develop their own ROV survey programs Examples include our collaboration with Makah Fisheries Management in Neah Bay who are interested in developing their own ROV program to monitor kelp forests and urchin barrens as well as the Tulalip who are interested in developing their own ROV program to monitor kelp It is our hope the methods we have developed will enable numerous entities throughout the Salish Sea and beyond to increase the

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text:
amount of data being gathered in support of kelp forest conservation and restoration Monitoring Puget Sound kelp forests with unmanned systems and artificial intelligence Principal Investigator PI Shawn Larson Curator of Conservation Research Seattle Aquarium 1484 Alaskan Way Seattle Washington 98101 206 618 3762 Co Investigators Zachary Randell1 Bob Pacunski2 Jodie Toft3 Jenny Waddell4 Blake Feist5 Seattle Aquarium1 Washington Department of Fish and Wildlife2 Puget Sound Restoration Fund3 Olympic Coast National Marine Sanctuary4 National Marine Fisheries Service5 Conservation Need Kelp forests in Washington provide habitat and refuge to numerous culturally important species and those of significant management concern including state federally and internationally listed threatened and endangered species such as pinto abalone Haliotis kamtschatkana sunflower sea star Pycnopodia helianthoides yelloweye and bocaccio rockfish Sebastes ruberrimus and Sebastes paucispinis respectively and several stocks of salmon Understanding the distribution functioning and overall health of Washington s coastal kelp forests is vital for supporting regional management and conservation of coastal resources particularly within the context of changing ocean conditions A central challenge to coastal management and conservation is the inability to survey the seafloor across a broad spatial extent Scientific SCUBA divers are readily able to survey relatively small areas on the order

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text:
of 10 100s of meters but as kelp forests exhibit high spatial variability and rich spatiotemporal dynamics inference from a few locations cannot easily be generalized out to broader scales Remotely operated vehicles ROVs have the potential to capture data across a comparatively large spatial extent but thus far their size and expense has mostly restricted ROV usage to exploring deep locations unsuitable for divers However technological advancement has produced ROV models that are small affordable capture high resolution imagery and are deployable from small vessels Abstract In conjunction with state federal and non profit partners we propose to bring the next generation of monitoring technology including unmanned systems and artificial intelligence AI algorithms into the kelp forests of Puget Sound Specifically we propose to develop standardized survey protocols to obtain high resolution video of the benthos via a small maneuverable ROV Given the volume of data we intend to collect it would be impractical for human observers to annotate all the imagery Instead we will harness advancements in AI specifically machine learning methods such as supervised classification to 1 identify individual and conspicuous objects such as purple urchins and sea stars and 2 obtain percent coverage estimates for colonial

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text:
and sessile taxa from photographic stills The Seattle Aquarium was recently awarded a grant to purchase the a small customizable ROV On the underside of this ROV we will stage two image stabilized cameras light strobes and laser size guides Next we will scrutinize the efficacy ROV operations in kelp forests This will be challenging the tether will likely tangle at times in kelp and murky water will obscure images but by collaborating with state and federal researchers already engaged in ROV work we believe our combined efforts will provide the best chance at overcoming these challenges In conjunction with Puget Sound Restoration Fund PSRF we will conduct ROV surveys along their long term subtidal index sites in Puget Sound This will allow us to explicitly contrast how taxonomic resolution varies between ROV obtained images processed via machine learning versus data collected via divers This will also allow us to determine if the ROV images are able to detect cryptic taxa such as pinto abalone And via a ROV acoustic tracking system from Washington Department of Fish and Wildlife WDFW our processed imagery will include geospatial coordinates enabling our data to be combined with other layers to generate habitat suitability

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